Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   F7R26_RS02310 Genome accession   NZ_CP062803
Coordinates   513216..514691 (-) Length   491 a.a.
NCBI ID   WP_043343436.1    Uniprot ID   A0A643FNT8
Organism   Cupriavidus basilensis strain DSM 11853     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508216..519691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7R26_RS02280 (F7R26_002275) - 508678..509355 (-) 678 WP_150991824.1 LON peptidase substrate-binding domain-containing protein -
  F7R26_RS02285 (F7R26_002280) rapZ 509422..510339 (-) 918 WP_150991821.1 RNase adapter RapZ -
  F7R26_RS02290 (F7R26_002285) - 510459..510782 (-) 324 WP_150991818.1 PsiF family protein -
  F7R26_RS02295 (F7R26_002290) hprK 510971..511942 (-) 972 WP_006159437.1 HPr(Ser) kinase/phosphatase -
  F7R26_RS02300 (F7R26_002295) ptsN 511978..512433 (-) 456 WP_006159436.1 PTS IIA-like nitrogen regulatory protein PtsN -
  F7R26_RS02305 (F7R26_002300) hpf 512816..513166 (-) 351 WP_006159435.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  F7R26_RS02310 (F7R26_002305) rpoN/rpoN1 513216..514691 (-) 1476 WP_043343436.1 RNA polymerase factor sigma-54 Machinery gene
  F7R26_RS02315 (F7R26_002310) lptB 514827..515612 (-) 786 WP_150991814.1 LPS export ABC transporter ATP-binding protein -
  F7R26_RS02320 (F7R26_002315) lptA 515637..516281 (-) 645 WP_150991812.1 lipopolysaccharide transport periplasmic protein LptA -
  F7R26_RS02325 (F7R26_002320) lptC 516336..516962 (-) 627 WP_150991856.1 LPS export ABC transporter periplasmic protein LptC -
  F7R26_RS02330 (F7R26_002325) - 516980..517963 (-) 984 WP_150991810.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54787.14 Da        Isoelectric Point: 4.9180

>NTDB_id=491471 F7R26_RS02310 WP_043343436.1 513216..514691(-) (rpoN/rpoN1) [Cupriavidus basilensis strain DSM 11853]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWLDSPLRVSSDGSVNLQSAPAPAPADGQ
GTSENRPDGAPEADDSYSDSNEDYGSDWSLDDFARRPQGEDDEKTPMQLRDAEPTLREHLMEQLAPLKISLRDKGLAIFL
VESLDDDGYLSTTLEEICSELPEELEFEVDEIQAVLTLLQSFDPPGVGARNAAECLALQLRRLKHPQRELALSIVTNHLE
LLAVRDYTRLKKALQVDEPELKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKLSGGWIAHLNPDVMPRLRINDMYAQ
ILRGAKGDAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFTHGEIAMRPLVLREIADTLGLHESTISRVT
TNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDARNPLSDSRIAELLGEQGFVVARRTVAKYREAL
KIPAVNLRKTL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=491471 F7R26_RS02310 WP_043343436.1 513216..514691(-) (rpoN/rpoN1) [Cupriavidus basilensis strain DSM 11853]
ATGAAACCGTCGCTACAGCTCCGCCTCTCGCAGCATCTCGCGCTTACGCCGCAACTCCAGCAGTCGATCCGGCTGCTGCA
ACTGTCCACGCTTGAACTGCAGCAGGAAGTGGAACAGGCGCTGACCGAGAACCCCTTGCTCGAGCGCGAGAACGACTGGC
TGGACAGCCCGCTGCGCGTATCTTCGGACGGCTCGGTGAACCTGCAGAGCGCGCCAGCGCCGGCACCCGCCGATGGCCAG
GGCACCAGCGAGAACCGCCCCGATGGCGCCCCCGAGGCCGACGACAGCTACTCCGACAGCAATGAGGACTACGGCAGCGA
CTGGAGCCTGGACGACTTCGCCCGCCGCCCGCAAGGCGAAGACGACGAAAAAACCCCGATGCAGCTGCGCGACGCCGAGC
CCACGCTGCGTGAACACCTCATGGAGCAGCTGGCGCCGCTCAAGATTTCCCTGCGCGACAAAGGGCTGGCTATCTTCCTG
GTCGAATCCCTGGACGATGACGGCTACCTGAGCACCACGCTCGAGGAAATCTGCAGCGAGCTGCCGGAGGAGCTCGAGTT
CGAGGTCGACGAGATCCAGGCTGTGCTCACGCTGCTGCAGAGCTTCGATCCGCCGGGCGTCGGGGCGCGCAATGCCGCCG
AGTGCCTGGCGCTGCAATTGCGCAGGCTCAAGCATCCGCAGCGCGAACTGGCGCTGAGCATCGTCACCAACCACCTCGAG
CTGCTGGCGGTGCGCGATTACACGCGCCTGAAAAAAGCGCTGCAAGTCGACGAGCCGGAACTCAAGGCAGCACATGAACT
GATCCGCTCGCTCGCGCCCTACCCCGGCCATGCCTACAGCCGGCCAGAAGCGGACTTCGTGGTGCCCGATGTTTTTGTTC
GAAAGTTGAGCGGCGGATGGATCGCCCACCTCAACCCGGATGTGATGCCACGGCTACGCATCAACGACATGTACGCGCAG
ATTTTGCGCGGTGCAAAAGGCGATGCGGGCGCGGCCGGACTGCAGCAAAAGCTGCAGGAAGCACGTTGGTTGATCAAGAA
TATCCAGCAAAGGTTCGATACGATATTGCGCGTCGCACAAGCCATTGTCGAACGTCAAAAGAACTTCTTCACCCACGGTG
AAATTGCGATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCCACCATCTCTCGGGTGACT
ACCAATAAATACATGGCAACGCCGATGGGGACTTTCGAGCTGAAGTATTTTTTCGGCTCTCACGTATCCACAGAAACCGG
CGGCGCGGCCTCATCAACGGCAATCCGTGCGTTGATCAAGCAATTGATAGGAGCCGAAGACGCTAGGAACCCCTTGTCCG
ACAGCCGGATTGCCGAATTGCTCGGCGAGCAAGGCTTTGTGGTAGCACGGCGTACCGTTGCCAAGTACCGCGAAGCGCTC
AAGATTCCCGCGGTCAATCTCCGTAAGACTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(334-489)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-318)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A643FNT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

78.758

100

0.8