Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KG092_RS01980 Genome accession   NZ_CP074366
Coordinates   427970..428437 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain ZYB62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422970..433437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KG092_RS01955 (KG092_01925) rpsG 423111..423581 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  KG092_RS01960 (KG092_01930) fusA 423678..425792 (+) 2115 WP_000124700.1 elongation factor G -
  KG092_RS01965 (KG092_01935) tuf 425863..427047 (+) 1185 WP_000031783.1 elongation factor Tu -
  KG092_RS01970 (KG092_01940) bfd 427230..427424 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  KG092_RS01975 (KG092_01945) bfr 427497..427973 (+) 477 WP_000675515.1 bacterioferritin -
  KG092_RS01980 (KG092_01950) pilD 427970..428437 (-) 468 WP_000340171.1 prepilin peptidase Machinery gene
  KG092_RS01985 (KG092_01955) rpsJ 428816..429127 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  KG092_RS01990 (KG092_01960) rplC 429160..429789 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  KG092_RS01995 (KG092_01965) rplD 429800..430405 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  KG092_RS02000 (KG092_01970) rplW 430402..430704 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  KG092_RS02005 (KG092_01975) rplB 430722..431543 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  KG092_RS02010 (KG092_01980) rpsS 431560..431838 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  KG092_RS02015 (KG092_01985) rplV 431853..432185 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  KG092_RS02020 (KG092_01990) rpsC 432203..432904 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  KG092_RS02025 (KG092_01995) rplP 432917..433327 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=490986 KG092_RS01980 WP_000340171.1 427970..428437(-) (pilD) [Escherichia coli strain ZYB62]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=490986 KG092_RS01980 WP_000340171.1 427970..428437(-) (pilD) [Escherichia coli strain ZYB62]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361