Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KGQ53_RS16460 Genome accession   NZ_CP074120
Coordinates   3469359..3470036 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain EC16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3464359..3475036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGQ53_RS16445 chiA 3465726..3468419 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KGQ53_RS16450 bfd 3468588..3468782 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KGQ53_RS16455 bfr 3468854..3469330 (+) 477 WP_000675504.1 bacterioferritin -
  KGQ53_RS16460 pilD 3469359..3470036 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KGQ53_RS16465 gspM 3470036..3470497 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KGQ53_RS16470 gspL 3470494..3471657 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KGQ53_RS16475 gspK 3471672..3472655 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KGQ53_RS16480 gspJ 3472648..3473235 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KGQ53_RS16485 gspI 3473228..3473605 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KGQ53_RS16490 gspH 3473602..3474111 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  KGQ53_RS16495 gspG 3474119..3474556 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=490715 KGQ53_RS16460 WP_000178161.1 3469359..3470036(-) (pilD) [Escherichia coli strain EC16]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=490715 KGQ53_RS16460 WP_000178161.1 3469359..3470036(-) (pilD) [Escherichia coli strain EC16]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378