Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   KFU85_RS09565 Genome accession   NZ_CP074052
Coordinates   1999723..2001150 (-) Length   475 a.a.
NCBI ID   WP_072664921.1    Uniprot ID   -
Organism   Escherichia coli strain PD23     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1994723..2006150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU85_RS09530 (KFU85_09465) hisL 1995307..1995357 (-) 51 WP_001364200.1 his operon leader peptide -
  KFU85_RS09535 (KFU85_09470) yefM 1995640..1995891 (+) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  KFU85_RS09540 (KFU85_09475) yoeB 1995888..1996142 (+) 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  KFU85_RS09545 (KFU85_09480) yeeZ 1996225..1997049 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  KFU85_RS09550 (KFU85_09485) yeeY 1997095..1998024 (+) 930 WP_000803363.1 LysR substrate-binding domain-containing protein -
  KFU85_RS09555 (KFU85_09490) yoeI 1998239..1998301 (+) 63 WP_010723108.1 membrane protein YoeI -
  KFU85_RS09560 (KFU85_09495) plaP 1998291..1999649 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  KFU85_RS09565 (KFU85_09500) sbcB 1999723..2001150 (-) 1428 WP_072664921.1 exodeoxyribonuclease I Machinery gene
  KFU85_RS09570 (KFU85_09505) dacD 2001359..2002525 (+) 1167 WP_088145596.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  KFU85_RS09575 (KFU85_09510) sbmC 2002644..2003117 (+) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  KFU85_RS09580 (KFU85_09515) yeeA 2003315..2004373 (+) 1059 WP_001200890.1 FUSC family protein -
  KFU85_RS09585 (KFU85_09520) tmaR 2004545..2004874 (+) 330 WP_000450409.1 DUF496 family protein -
  KFU85_RS09590 (KFU85_09525) - 2004975..2005418 (-) 444 WP_063815002.1 EutP/PduV family microcompartment system protein -
  KFU85_RS09595 (KFU85_09530) pduU 2005423..2005773 (-) 351 WP_000211397.1 propanediol utilization microcompartment protein PduU -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54467.65 Da        Isoelectric Point: 5.2416

>NTDB_id=490522 KFU85_RS09565 WP_072664921.1 1999723..2001150(-) (sbcB) [Escherichia coli strain PD23]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
ERLGIDRQHCLDNLKVLRENPQVRDKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDDNEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=490522 KFU85_RS09565 WP_072664921.1 1999723..2001150(-) (sbcB) [Escherichia coli strain PD23]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAAC
GAAGCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAGCTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCACATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGTCCGGAAGACGCT
GAGCGTTTAGGGATTGATCGTCAACATTGCCTGGATAACCTGAAAGTGTTGCGCGAAAACCCGCAAGTACGCGACAAAGT
TGTGGCAATTTTTGCAGAAGCTGAACCGTTTACACCATCAGATAATGTAGATGCACAGCTTTATAATGGCTTTTTTAGCG
ACGCCGATCGTGCAGCAATGAAAATCGTGCTGGAGACTGAACCGCGTAATTTACCGGCTCTGGATATCACCTTTGTTGAT
AAGCGTATCGAGAAACTCCTGTTCAACTACCGCGCCCGCAACTTCCCCGGCACGTTAGATGACAACGAGCAGCAACGCTG
GCTTGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.362

98.947

0.587