Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   ILP76_RS00835 Genome accession   NZ_CP062469
Coordinates   195597..197225 (+) Length   542 a.a.
NCBI ID   WP_061642462.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MRSA - AMRF 4     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 198167..208066 195597..197225 flank 942
SCCmec 165700..196926 195597..197225 flank -1329


Gene organization within MGE regions


Location: 165700..208066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ILP76_RS00700 (ILP76_00700) - 165700..166854 (-) 1155 WP_000912116.1 ATP-binding protein -
  ILP76_RS00705 (ILP76_00705) - 167309..168355 (+) 1047 WP_001042023.1 hypothetical protein -
  ILP76_RS00710 (ILP76_00710) ssb 168548..168844 (+) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  ILP76_RS00715 (ILP76_00715) cch1 168844..170637 (+) 1794 WP_000079473.1 cassette chromosome replicative helicase -
  ILP76_RS00720 (ILP76_00720) ccrA 170871..172220 (+) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  ILP76_RS00725 (ILP76_00725) - 172388..173557 (+) 1170 Protein_146 IS256-like element IS256 family transposase -
  ILP76_RS00730 (ILP76_00730) aph(2'')-Ia 173687..175126 (-) 1440 WP_001028144.1 aminoglycoside O-phosphotransferase APH(2'')-Ia -
  ILP76_RS00735 (ILP76_00735) - 175127..175519 (-) 393 WP_000393259.1 GNAT family N-acetyltransferase -
  ILP76_RS14875 - 175576..175860 (-) 285 WP_000581058.1 IS256 family transposase -
  ILP76_RS00740 (ILP76_00740) - 175857..176746 (-) 890 Protein_150 IS256 family transposase -
  ILP76_RS00745 (ILP76_00745) ccrB 176802..178439 (+) 1638 WP_194085687.1 cassette chromosome recombinase CcrB Machinery gene
  ILP76_RS00750 (ILP76_00750) - 178961..179311 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ILP76_RS00755 (ILP76_00755) - 179398..179709 (+) 312 WP_000700851.1 DUF960 family protein -
  ILP76_RS00760 (ILP76_00760) - 179724..180230 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  ILP76_RS00765 (ILP76_00765) - 180279..181889 (+) 1611 Protein_155 IS1182 family transposase -
  ILP76_RS00770 (ILP76_00770) - 181880..182122 (-) 243 Protein_156 type I restriction enzyme endonuclease domain-containing protein -
  ILP76_RS00775 (ILP76_00775) - 182112..183098 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  ILP76_RS00780 (ILP76_00780) mecA 183195..185204 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  ILP76_RS00785 (ILP76_00785) - 185250..185678 (-) 429 WP_000872606.1 MaoC family dehydratase -
  ILP76_RS00790 (ILP76_00790) - 185775..186518 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  ILP76_RS00795 (ILP76_00795) - 187394..187783 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  ILP76_RS00800 (ILP76_00800) - 187819..188492 (+) 674 WP_228076481.1 IS6-like element IS257 family transposase -
  ILP76_RS00805 (ILP76_00805) - 188539..188709 (+) 171 WP_155734542.1 hypothetical protein -
  ILP76_RS00810 (ILP76_00810) - 188742..189653 (-) 912 WP_046464619.1 ABC transporter substrate-binding protein -
  ILP76_RS00815 (ILP76_00815) - 190284..191798 (+) 1515 WP_046464618.1 hypothetical protein -
  ILP76_RS00820 (ILP76_00820) ssb 191969..192262 (+) 294 WP_046464617.1 cassette chromosome ssDNA-binding protein -
  ILP76_RS00825 (ILP76_00825) cch1 192262..194028 (+) 1767 WP_061544227.1 cassette chromosome replicative helicase -
  ILP76_RS00830 (ILP76_00830) ccrA 194239..195600 (+) 1362 WP_061544228.1 cassette chromosome recombinase CcrA Machinery gene
  ILP76_RS00835 (ILP76_00835) ccrB 195597..197225 (+) 1629 WP_061642462.1 cassette chromosome recombinase CcrB Machinery gene
  ILP76_RS00840 (ILP76_00840) - 197730..198080 (+) 351 WP_061544230.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ILP76_RS00845 (ILP76_00845) - 198167..198478 (+) 312 WP_061544231.1 DUF960 family protein -
  ILP76_RS00850 (ILP76_00850) - 198493..198996 (+) 504 WP_037559702.1 DUF1643 domain-containing protein -
  ILP76_RS00855 (ILP76_00855) - 199011..199232 (+) 222 WP_061544232.1 hypothetical protein -
  ILP76_RS00860 (ILP76_00860) - 199433..199528 (+) 96 WP_099560939.1 type I toxin-antitoxin system Fst family toxin -
  ILP76_RS14880 - 199898..200449 (-) 552 WP_416222490.1 restriction endonuclease subunit S -
  ILP76_RS14885 - 200473..200851 (-) 379 Protein_176 restriction endonuclease subunit S -
  ILP76_RS00870 (ILP76_00870) - 201090..202259 (-) 1170 Protein_177 IS256 family transposase -
  ILP76_RS14735 - 202337..202468 (-) 132 WP_265588472.1 hypothetical protein -
  ILP76_RS00875 (ILP76_00875) - 202461..204017 (-) 1557 WP_194085653.1 type I restriction-modification system subunit M -
  ILP76_RS00880 (ILP76_00880) - 204018..204599 (-) 582 WP_046044628.1 restriction endonuclease subunit S -
  ILP76_RS00885 (ILP76_00885) - 204689..207481 (-) 2793 WP_046044629.1 type I restriction endonuclease subunit R -
  ILP76_RS00890 (ILP76_00890) rlmH 207587..208066 (-) 480 WP_089418590.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62339.47 Da        Isoelectric Point: 9.5866

>NTDB_id=490268 ILP76_RS00835 WP_061642462.1 195597..197225(+) (ccrB) [Staphylococcus aureus strain MRSA - AMRF 4]
MTQLKKKRIGLYARVSTEMQTDGYSIHGQLNQLTEYCQFQGYEVVDEYTDRGISGKTTQRPELQRMLKDAKGGKLDGIMV
YNTTRLARNTSDLLNIVEELYEINVEFFSLTEKIEIASSTGKLMLQILASFAEFERNVIVENVFNGQRQRAIEGYYQGNL
PLGYDKVPESKKELMINQHEANIVKYIFESYAKGHGYRKIANTLNHKGYVTKKGKPFSISSITYIISNPFYIGKIQFAKY
RHWSDKRRKGLNEEPIIADGKHAPIIDKALWDKVQFKRQESRKKPQVHGKGTNLLTGIVKCPKCGAAMAASNTTNTLKDG
TKKRIRYYSCSNFRNKGSKVCSANSVRADVLEKYVMDQILEIIKSKKVLKQLVEKVNEQSQIDKSSLNHDIAYKQSQCEE
LKAKMHTLTKTIEDSPDLDSVLKPTILNYQDELNQINNQIHQLEQDKQAEAPHYDADMIANVLQAIFKDIDKLEKSQLKS
LYLTVIDRIDIRNHEHHKKQFYVTLKLNNEIIKQLFNNRPLDEVLLSTSSLFLPQTIYITIE

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=490268 ILP76_RS00835 WP_061642462.1 195597..197225(+) (ccrB) [Staphylococcus aureus strain MRSA - AMRF 4]
ATGACACAACTCAAAAAGAAACGTATTGGATTATACGCACGTGTATCAACTGAAATGCAAACGGATGGTTACAGTATCCA
TGGTCAATTAAATCAACTTACAGAGTATTGTCAATTTCAAGGTTATGAAGTCGTTGATGAATATACCGATCGTGGTATAT
CAGGTAAAACAACGCAACGTCCTGAACTACAACGTATGTTAAAAGATGCTAAAGGAGGTAAATTAGATGGTATTATGGTA
TATAATACTACTCGTTTAGCTAGAAATACCTCAGATTTATTAAATATTGTCGAAGAATTGTATGAGATTAATGTTGAATT
TTTTAGTCTAACTGAAAAAATAGAAATTGCGAGCTCCACAGGTAAGTTGATGCTCCAAATCTTAGCGAGCTTCGCAGAAT
TTGAAAGAAACGTTATTGTAGAAAACGTGTTTAATGGCCAAAGACAACGTGCTATTGAAGGTTATTACCAAGGTAATTTA
CCTTTAGGCTATGATAAAGTACCTGAGAGTAAAAAAGAGCTTATGATTAATCAACATGAAGCAAATATTGTTAAATATAT
TTTTGAGTCATACGCCAAAGGTCACGGTTATCGTAAGATTGCGAACACATTGAATCATAAAGGTTATGTGACTAAGAAAG
GGAAACCGTTTAGTATTTCGTCCATTACTTATATCATCTCAAACCCTTTTTACATTGGAAAGATACAGTTTGCGAAGTAT
AGACACTGGAGCGATAAAAGACGAAAAGGACTCAATGAGGAACCGATTATCGCAGATGGTAAGCATGCGCCTATTATTGA
TAAAGCTTTATGGGATAAAGTACAGTTTAAACGCCAAGAGAGTCGCAAGAAACCACAAGTTCATGGTAAAGGGACCAATC
TACTTACTGGGATTGTAAAGTGCCCAAAATGTGGCGCAGCCATGGCGGCAAGTAATACGACCAATACATTAAAAGATGGC
ACTAAGAAAAGAATCAGATATTATTCATGTAGCAATTTTAGAAACAAAGGTTCTAAAGTATGTTCAGCCAATAGTGTTAG
AGCAGATGTATTAGAAAAATATGTTATGGACCAGATATTGGAAATTATCAAAAGTAAAAAGGTATTGAAGCAATTGGTTG
AAAAAGTAAATGAACAAAGCCAAATAGATAAATCTTCTTTGAATCATGATATTGCCTATAAACAGTCACAATGTGAAGAA
CTAAAAGCCAAAATGCATACATTAACGAAAACCATTGAAGACTCACCTGACTTAGATAGCGTATTGAAACCAACAATACT
TAACTATCAAGATGAATTGAATCAAATAAATAATCAAATCCATCAACTAGAGCAAGATAAACAAGCAGAAGCTCCACATT
ATGATGCTGATATGATTGCCAACGTACTACAAGCAATATTCAAAGATATAGACAAATTAGAGAAATCTCAGTTGAAATCA
CTCTATCTGACGGTTATCGATCGTATTGATATCCGAAACCATGAACATCATAAGAAGCAATTTTATGTTACGCTTAAACT
GAATAATGAAATTATCAAACAACTATTTAATAACCGTCCACTTGACGAAGTGCTCCTTAGCACTTCGTCTTTATTTTTGC
CACAAACTATATATATAACTATTGAATAA

Domains

Predicted by InterproScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(300-355)

Resolvase, N terminal domain (Resolvase)

(11-154)

Recombinase (Recombinase)

(179-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

72.14

100

0.721