Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   ILP76_RS00720 Genome accession   NZ_CP062469
Coordinates   170871..172220 (+) Length   449 a.a.
NCBI ID   WP_000815640.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MRSA - AMRF 4     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 165700..196926 170871..172220 within 0
IScluster/Tn 172388..188208 170871..172220 flank 168


Gene organization within MGE regions


Location: 165700..196926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ILP76_RS00700 (ILP76_00700) - 165700..166854 (-) 1155 WP_000912116.1 ATP-binding protein -
  ILP76_RS00705 (ILP76_00705) - 167309..168355 (+) 1047 WP_001042023.1 hypothetical protein -
  ILP76_RS00710 (ILP76_00710) ssb 168548..168844 (+) 297 WP_000273823.1 cassette chromosome ssDNA-binding protein -
  ILP76_RS00715 (ILP76_00715) cch1 168844..170637 (+) 1794 WP_000079473.1 cassette chromosome replicative helicase -
  ILP76_RS00720 (ILP76_00720) ccrA 170871..172220 (+) 1350 WP_000815640.1 cassette chromosome recombinase CcrA Machinery gene
  ILP76_RS00725 (ILP76_00725) - 172388..173557 (+) 1170 Protein_146 IS256-like element IS256 family transposase -
  ILP76_RS00730 (ILP76_00730) aph(2'')-Ia 173687..175126 (-) 1440 WP_001028144.1 aminoglycoside O-phosphotransferase APH(2'')-Ia -
  ILP76_RS00735 (ILP76_00735) - 175127..175519 (-) 393 WP_000393259.1 GNAT family N-acetyltransferase -
  ILP76_RS14875 - 175576..175860 (-) 285 WP_000581058.1 IS256 family transposase -
  ILP76_RS00740 (ILP76_00740) - 175857..176746 (-) 890 Protein_150 IS256 family transposase -
  ILP76_RS00745 (ILP76_00745) ccrB 176802..178439 (+) 1638 WP_194085687.1 cassette chromosome recombinase CcrB Machinery gene
  ILP76_RS00750 (ILP76_00750) - 178961..179311 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  ILP76_RS00755 (ILP76_00755) - 179398..179709 (+) 312 WP_000700851.1 DUF960 family protein -
  ILP76_RS00760 (ILP76_00760) - 179724..180230 (+) 507 WP_001092189.1 DUF1643 domain-containing protein -
  ILP76_RS00765 (ILP76_00765) - 180279..181889 (+) 1611 Protein_155 IS1182 family transposase -
  ILP76_RS00770 (ILP76_00770) - 181880..182122 (-) 243 Protein_156 type I restriction enzyme endonuclease domain-containing protein -
  ILP76_RS00775 (ILP76_00775) - 182112..183098 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  ILP76_RS00780 (ILP76_00780) mecA 183195..185204 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  ILP76_RS00785 (ILP76_00785) - 185250..185678 (-) 429 WP_000872606.1 MaoC family dehydratase -
  ILP76_RS00790 (ILP76_00790) - 185775..186518 (-) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  ILP76_RS00795 (ILP76_00795) - 187394..187783 (-) 390 WP_020444595.1 hydroxymethylglutaryl-CoA synthase -
  ILP76_RS00800 (ILP76_00800) - 187819..188492 (+) 674 WP_228076481.1 IS6-like element IS257 family transposase -
  ILP76_RS00805 (ILP76_00805) - 188539..188709 (+) 171 WP_155734542.1 hypothetical protein -
  ILP76_RS00810 (ILP76_00810) - 188742..189653 (-) 912 WP_046464619.1 ABC transporter substrate-binding protein -
  ILP76_RS00815 (ILP76_00815) - 190284..191798 (+) 1515 WP_046464618.1 hypothetical protein -
  ILP76_RS00820 (ILP76_00820) ssb 191969..192262 (+) 294 WP_046464617.1 cassette chromosome ssDNA-binding protein -
  ILP76_RS00825 (ILP76_00825) cch1 192262..194028 (+) 1767 WP_061544227.1 cassette chromosome replicative helicase -
  ILP76_RS00830 (ILP76_00830) ccrA 194239..195600 (+) 1362 WP_061544228.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52663.34 Da        Isoelectric Point: 9.9639

>NTDB_id=490265 ILP76_RS00720 WP_000815640.1 170871..172220(+) (ccrA) [Staphylococcus aureus strain MRSA - AMRF 4]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYEYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRLQKQTKQTPSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=490265 ILP76_RS00720 WP_000815640.1 170871..172220(+) (ccrA) [Staphylococcus aureus strain MRSA - AMRF 4]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGAATATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGTCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACACCTTCGGATAACCAACTAAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.947

100

0.759