Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   KFU70_RS00515 Genome accession   NZ_CP074033
Coordinates   103316..104743 (-) Length   475 a.a.
NCBI ID   WP_000980570.1    Uniprot ID   -
Organism   Escherichia coli strain PI7     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 98316..109743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU70_RS00485 (KFU70_00485) yeeZ 98371..99195 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  KFU70_RS00490 (KFU70_00490) yeeY 99241..100170 (+) 930 WP_000803360.1 LysR substrate-binding domain-containing protein -
  KFU70_RS00495 (KFU70_00495) yoeI 100385..100447 (+) 63 WP_010723108.1 membrane protein YoeI -
  KFU70_RS00500 (KFU70_00500) plaP 100437..101795 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  KFU70_RS00505 (KFU70_00505) tsuA 101974..103032 (+) 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
  KFU70_RS00510 (KFU70_00510) tsuB 103046..103273 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  KFU70_RS00515 (KFU70_00515) sbcB 103316..104743 (-) 1428 WP_000980570.1 exodeoxyribonuclease I Machinery gene
  KFU70_RS00520 (KFU70_00520) dacD 104952..106118 (+) 1167 WP_001320295.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  KFU70_RS00525 (KFU70_00525) sbmC 106237..106710 (+) 474 WP_001105393.1 DNA gyrase inhibitor SbmC -
  KFU70_RS00530 (KFU70_00530) yeeA 106908..107966 (+) 1059 WP_001200905.1 FUSC family protein -
  KFU70_RS00535 (KFU70_00535) tmaR 108138..108467 (+) 330 WP_000450409.1 DUF496 family protein -
  KFU70_RS00540 (KFU70_00540) - 108568..108750 (-) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  KFU70_RS00545 (KFU70_00545) - 108813..108950 (+) 138 Protein_108 transposase domain-containing protein -
  KFU70_RS00550 (KFU70_00550) - 109239..109331 (-) 93 Protein_109 DUF957 domain-containing protein -
  KFU70_RS00555 (KFU70_00555) yeeW 109365..109559 (-) 195 WP_000988599.1 DUF5983 family protein -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54529.81 Da        Isoelectric Point: 5.4720

>NTDB_id=490255 KFU70_RS00515 WP_000980570.1 103316..104743(-) (sbcB) [Escherichia coli strain PI7]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EATFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=490255 KFU70_RS00515 WP_000980570.1 103316..104743(-) (sbcB) [Escherichia coli strain PI7]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCACGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCAGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCCGATCGCGCAGCAATGAAAATTGTGCTGGAAACCGAGCCACGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTATTCACACCCGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGT
ATGCCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAG

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.723

98.947

0.581