Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU81_RS02070 Genome accession   NZ_CP074012
Coordinates   449977..450654 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain PI24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444977..455654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU81_RS02050 (KFU81_02020) - 445395..446092 (+) 698 WP_103215986.1 IS1-like element IS1A family transposase -
  KFU81_RS02055 (KFU81_02025) chiA 446344..449037 (+) 2694 WP_053882639.1 bifunctional chitinase/lysozyme -
  KFU81_RS02060 (KFU81_02030) bfd 449206..449400 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU81_RS02065 (KFU81_02035) bfr 449472..449948 (+) 477 WP_000675504.1 bacterioferritin -
  KFU81_RS02070 (KFU81_02040) pilD 449977..450654 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KFU81_RS02075 (KFU81_02045) gspM 450654..451115 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU81_RS02080 (KFU81_02050) gspL 451112..452275 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  KFU81_RS02085 (KFU81_02055) gspK 452290..453273 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU81_RS02090 (KFU81_02060) gspJ 453266..453853 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU81_RS02095 (KFU81_02065) gspI 453846..454223 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU81_RS02100 (KFU81_02070) gspH 454220..454729 (-) 510 WP_257163062.1 type II secretion system minor pseudopilin GspH -
  KFU81_RS02105 (KFU81_02075) gspG 454737..455174 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=489859 KFU81_RS02070 WP_000178161.1 449977..450654(-) (pilD) [Escherichia coli strain PI24]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=489859 KFU81_RS02070 WP_000178161.1 449977..450654(-) (pilD) [Escherichia coli strain PI24]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378