Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   KFU74_RS20710 Genome accession   NZ_CP074007
Coordinates   4323828..4325210 (-) Length   460 a.a.
NCBI ID   WP_001029692.1    Uniprot ID   -
Organism   Escherichia coli strain PD4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4318828..4330210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU74_RS20695 (KFU74_20560) ettA 4320600..4322267 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  KFU74_RS20700 (KFU74_20565) nadS 4322428..4322541 (-) 114 Protein_4066 NadS family protein -
  KFU74_RS20705 (KFU74_20570) nadR 4322575..4323807 (-) 1233 WP_000093812.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  KFU74_RS20710 (KFU74_20575) radA/sms 4323828..4325210 (-) 1383 WP_001029692.1 DNA repair protein RadA Machinery gene
  KFU74_RS20715 (KFU74_20580) serB 4325259..4326227 (-) 969 WP_001132955.1 phosphoserine phosphatase -
  KFU74_RS20720 (KFU74_20585) ytjB 4326333..4326977 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  KFU74_RS20725 (KFU74_20590) lplA 4327005..4328021 (+) 1017 WP_000105843.1 lipoate--protein ligase LplA -
  KFU74_RS20730 (KFU74_20595) yjjJ 4328022..4329353 (-) 1332 WP_016244252.1 type II toxin-antitoxin system HipA family toxin YjjJ -
  KFU74_RS20735 (KFU74_20600) tnpA 4329567..4330025 (-) 459 WP_257232810.1 IS200/IS605-like element IS200C family transposase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49456.04 Da        Isoelectric Point: 7.1936

>NTDB_id=489843 KFU74_RS20710 WP_001029692.1 4323828..4325210(-) (radA/sms) [Escherichia coli strain PD4]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADIQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=489843 KFU74_RS20710 WP_001029692.1 4323828..4325210(-) (radA/sms) [Escherichia coli strain PD4]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATTCAGGTGATGCATATGGCGGATAT
ACAATCATCGCCTGGCAGTGTGGCGCAGGTGCGTGAAACAGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGCTCGCTGGCTGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTCTGGATGGTGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAG
TGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCGGTAGGTCTGGAGCAAAACCGTCTGGCGATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTGACAGAAACCAGTGCCG
ATTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGGCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

47.046

99.348

0.467
  radA Streptococcus mitis SK321

44.079

99.13

0.437
  radA Streptococcus pneumoniae D39

43.64

99.13

0.433
  radA Streptococcus pneumoniae TIGR4

43.64

99.13

0.433
  radA Streptococcus mitis NCTC 12261

43.64

99.13

0.433
  radA Streptococcus pneumoniae R6

43.64

99.13

0.433
  radA Streptococcus pneumoniae Rx1

43.64

99.13

0.433