Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KFU74_RS02765 Genome accession   NZ_CP074007
Coordinates   578559..580055 (+) Length   498 a.a.
NCBI ID   WP_001040626.1    Uniprot ID   -
Organism   Escherichia coli strain PD4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 573559..585055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU74_RS02735 (KFU74_02675) tusA 573788..574033 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  KFU74_RS02740 (KFU74_02680) zntA 574135..576333 (-) 2199 WP_000106560.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  KFU74_RS02745 (KFU74_02685) yhhN 576407..577033 (-) 627 WP_000964724.1 lysoplasmalogenase -
  KFU74_RS02750 (KFU74_02690) yhhM 577174..577551 (+) 378 WP_000042884.1 DUF2500 domain-containing protein -
  KFU74_RS02755 (KFU74_02695) yhhL 577554..577823 (-) 270 WP_001295207.1 DUF1145 family protein -
  KFU74_RS02760 (KFU74_02700) rsmD 577813..578409 (-) 597 WP_000743202.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  KFU74_RS02765 (KFU74_02705) pilA 578559..580055 (+) 1497 WP_001040626.1 signal recognition particle-docking protein FtsY Machinery gene
  KFU74_RS02770 (KFU74_02710) ftsE 580058..580726 (+) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  KFU74_RS02775 (KFU74_02715) ftsX 580719..581777 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  KFU74_RS02780 (KFU74_02720) rpoH 582022..582876 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  KFU74_RS02785 (KFU74_02725) livJ 583147..584250 (+) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  KFU74_RS24475 - 584310..584409 (+) 100 Protein_541 hypothetical protein -
  KFU74_RS02790 (KFU74_02730) panM 584450..584833 (-) 384 WP_000778792.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54694.50 Da        Isoelectric Point: 4.1659

>NTDB_id=489788 KFU74_RS02765 WP_001040626.1 578559..580055(+) (pilA) [Escherichia coli strain PD4]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEHAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=489788 KFU74_RS02765 WP_001040626.1 578559..580055(+) (pilA) [Escherichia coli strain PD4]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCGGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAAGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGATGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378