Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU69_RS01915 Genome accession   NZ_CP074005
Coordinates   422013..422690 (-) Length   225 a.a.
NCBI ID   WP_152934014.1    Uniprot ID   -
Organism   Escherichia coli strain PI12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417013..427690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU69_RS01900 (KFU69_01870) chiA 418380..421073 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KFU69_RS01905 (KFU69_01875) bfd 421242..421436 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU69_RS01910 (KFU69_01880) bfr 421508..421984 (+) 477 WP_000675504.1 bacterioferritin -
  KFU69_RS01915 (KFU69_01885) pilD 422013..422690 (-) 678 WP_152934014.1 prepilin peptidase GspO Machinery gene
  KFU69_RS01920 (KFU69_01890) gspM 422690..423151 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU69_RS01925 (KFU69_01895) gspL 423148..424311 (-) 1164 Protein_385 type II secretion system protein GspL -
  KFU69_RS01930 (KFU69_01900) gspK 424326..425309 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU69_RS01935 (KFU69_01905) gspJ 425302..425889 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU69_RS01940 (KFU69_01910) gspI 425882..426259 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU69_RS01945 (KFU69_01915) gspH 426256..426765 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  KFU69_RS01950 (KFU69_01920) gspG 426773..427210 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.69 Da        Isoelectric Point: 7.9563

>NTDB_id=489701 KFU69_RS01915 WP_152934014.1 422013..422690(-) (pilD) [Escherichia coli strain PI12]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDSK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=489701 KFU69_RS01915 WP_152934014.1 422013..422690(-) (pilD) [Escherichia coli strain PI12]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTCCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378