Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU72_RS02110 Genome accession   NZ_CP073994
Coordinates   457446..458123 (-) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain MB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452446..463123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU72_RS02095 (KFU72_02075) chiA 453813..456506 (+) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  KFU72_RS02100 (KFU72_02080) bfd 456675..456869 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU72_RS02105 (KFU72_02085) bfr 456941..457417 (+) 477 WP_000675504.1 bacterioferritin -
  KFU72_RS02110 (KFU72_02090) pilD 457446..458123 (-) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  KFU72_RS02115 (KFU72_02095) gspM 458123..458584 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU72_RS02120 (KFU72_02100) gspL 458581..459744 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  KFU72_RS02125 (KFU72_02105) gspK 459759..460742 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU72_RS02130 (KFU72_02110) gspJ 460735..461322 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU72_RS02135 (KFU72_02115) gspI 461315..461692 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU72_RS02140 (KFU72_02120) gspH 461689..462198 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  KFU72_RS02145 (KFU72_02125) gspG 462206..462643 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=489462 KFU72_RS02110 WP_001377644.1 457446..458123(-) (pilD) [Escherichia coli strain MB1]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=489462 KFU72_RS02110 WP_001377644.1 457446..458123(-) (pilD) [Escherichia coli strain MB1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373