Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   KFU65_RS08070 Genome accession   NZ_CP073988
Coordinates   1701656..1703080 (-) Length   474 a.a.
NCBI ID   WP_088845946.1    Uniprot ID   -
Organism   Escherichia coli strain MB10     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1696656..1708080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU65_RS08040 (KFU65_07980) yeeZ 1696711..1697535 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  KFU65_RS08045 (KFU65_07985) yeeY 1697581..1698510 (+) 930 WP_257204709.1 LysR substrate-binding domain-containing protein -
  KFU65_RS08050 (KFU65_07990) yoeI 1698725..1698787 (+) 63 WP_010723108.1 membrane protein YoeI -
  KFU65_RS08055 (KFU65_07995) plaP 1698777..1700135 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  KFU65_RS08060 (KFU65_08000) tsuA 1700314..1701372 (+) 1059 WP_000492340.1 thiosulfate utilization transporter TsuA/YeeE -
  KFU65_RS08065 (KFU65_08005) tsuB 1701386..1701613 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  KFU65_RS08070 (KFU65_08010) sbcB 1701656..1703080 (-) 1425 WP_088845946.1 exodeoxyribonuclease I Machinery gene
  KFU65_RS08075 (KFU65_08015) - 1703262..1704440 (+) 1179 WP_024239930.1 site-specific integrase -
  KFU65_RS08080 (KFU65_08020) - 1704421..1704612 (-) 192 WP_000132739.1 AlpA family transcriptional regulator -
  KFU65_RS08085 (KFU65_08025) - 1704694..1704993 (-) 300 WP_001303965.1 hypothetical protein -
  KFU65_RS08090 (KFU65_08030) - 1705065..1705349 (-) 285 WP_000002094.1 ASCH domain-containing protein -
  KFU65_RS08095 (KFU65_08035) - 1705342..1705890 (-) 549 WP_000207907.1 DUF551 domain-containing protein -
  KFU65_RS08100 (KFU65_08040) - 1705892..1706263 (-) 372 WP_077777070.1 ead/Ea22-like family protein -
  KFU65_RS08105 (KFU65_08045) - 1706305..1706526 (-) 222 WP_000763367.1 TraR/DksA family transcriptional regulator -
  KFU65_RS08110 (KFU65_08050) - 1706625..1706840 (-) 216 WP_000188857.1 hypothetical protein -
  KFU65_RS08115 (KFU65_08055) - 1706917..1707108 (-) 192 WP_024225579.1 DUF1382 family protein -
  KFU65_RS08120 (KFU65_08060) - 1707081..1707263 (-) 183 WP_000682298.1 DUF1317 domain-containing protein -
  KFU65_RS08125 (KFU65_08065) - 1707260..1707940 (-) 681 WP_050876072.1 lambda exonuclease family protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54381.63 Da        Isoelectric Point: 5.4720

>NTDB_id=489405 KFU65_RS08070 WP_088845946.1 1701656..1703080(-) (sbcB) [Escherichia coli strain MB10]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFNVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFEYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLNNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=489405 KFU65_RS08070 WP_088845946.1 1701656..1703080(-) (sbcB) [Escherichia coli strain MB10]
ATGACAGACACAGATAAACAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTGCCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAA
GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTAACGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGAATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGAATATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGACAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCAATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTCGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAGGGTTATGCAGATGAATTGCAGATGCTGGCACAACAATATG
CCGATGACAAAGAGAAAGTGGCACTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(211-471)

Exonuclease (RNase_T)

(12-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

99.156

0.584