Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   KFU92_RS10200 Genome accession   NZ_CP073984
Coordinates   2061543..2062553 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain MB15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2056543..2067553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU92_RS10170 (KFU92_10120) nudB 2058267..2058719 (+) 453 WP_077221229.1 dihydroneopterin triphosphate diphosphatase -
  KFU92_RS10175 (KFU92_10125) yebC 2058748..2059488 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  KFU92_RS10180 (KFU92_10130) ruvC 2059523..2060044 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  KFU92_RS10185 (KFU92_10135) yebB 2060046..2060648 (-) 603 WP_001024930.1 YebB family permuted papain-like enzyme -
  KFU92_RS10190 (KFU92_10140) yobI 2060719..2060784 (+) 66 WP_001386853.1 stress response small protein YobI -
  KFU92_RS10195 (KFU92_10145) ruvA 2060923..2061534 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  KFU92_RS10200 (KFU92_10150) ruvB 2061543..2062553 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  KFU92_RS10205 (KFU92_10155) znuB 2062700..2063485 (-) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  KFU92_RS10210 (KFU92_10160) znuC 2063482..2064237 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  KFU92_RS10215 (KFU92_10165) znuA 2064316..2065248 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  KFU92_RS10220 (KFU92_10170) mepM 2065264..2066586 (+) 1323 WP_121834110.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=489336 KFU92_RS10200 WP_000568519.1 2061543..2062553(+) (ruvB) [Escherichia coli strain MB15]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=489336 KFU92_RS10200 WP_000568519.1 2061543..2062553(+) (ruvB) [Escherichia coli strain MB15]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494