Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU73_RS16275 Genome accession   NZ_CP073974
Coordinates   3376843..3377520 (-) Length   225 a.a.
NCBI ID   WP_257182049.1    Uniprot ID   -
Organism   Escherichia coli strain MB22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3371843..3382520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU73_RS16260 (KFU73_16095) chiA 3373210..3375903 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KFU73_RS16265 (KFU73_16100) bfd 3376072..3376266 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU73_RS16270 (KFU73_16105) bfr 3376338..3376814 (+) 477 WP_000675504.1 bacterioferritin -
  KFU73_RS16275 (KFU73_16110) pilD 3376843..3377520 (-) 678 WP_257182049.1 prepilin peptidase GspO Machinery gene
  KFU73_RS16280 (KFU73_16115) gspM 3377520..3377981 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU73_RS16285 (KFU73_16120) gspL 3377978..3379141 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KFU73_RS16290 (KFU73_16125) gspK 3379156..3380139 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU73_RS16295 (KFU73_16130) gspJ 3380132..3380719 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU73_RS16300 (KFU73_16135) gspI 3380712..3381089 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU73_RS16305 (KFU73_16140) gspH 3381086..3381595 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  KFU73_RS16310 (KFU73_16145) gspG 3381603..3382040 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24912.77 Da        Isoelectric Point: 7.9563

>NTDB_id=489114 KFU73_RS16275 WP_257182049.1 3376843..3377520(-) (pilD) [Escherichia coli strain MB22]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACAPAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGTWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=489114 KFU73_RS16275 WP_257182049.1 3376843..3377520(-) (pilD) [Escherichia coli strain MB22]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCCGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCACATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378