Detailed information NTG0048880

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: RSPO_RS14500
• Genome accession: NC_017574
• Coordinates: 3145229..3146362 (+)
• Length: 377 a.a.
• NCBI ID: WP_014618044.1
• Uniprot ID: F6G4B5
• Organism: Ralstonia solanacearum Po82
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3140229..3151362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RSPO_RS14475 (RSPO_c03014)	-	3140597..3140845 (-)	249	WP_003262548.1	YfhL family 4Fe-4S dicluster ferredoxin	-
RSPO_RS14480 (RSPO_c03015)	coaD	3140896..3141402 (-)	507	WP_003262550.1	pantetheine-phosphate adenylyltransferase	-
RSPO_RS14485 (RSPO_c03016)	rsmD	3141598..3142254 (-)	657	WP_014618041.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
RSPO_RS14490 (RSPO_c03017)	-	3142332..3143675 (-)	1344	WP_014618042.1	M16 family metallopeptidase	-
RSPO_RS14495	-	3143675..3145168 (-)	1494	WP_039550994.1	M16 family metallopeptidase	-
RSPO_RS14500 (RSPO_c03018)	pilA	3145229..3146362 (+)	1134	WP_014618044.1	signal recognition particle-docking protein FtsY	Machinery gene
RSPO_RS14505 (RSPO_c03020)	maiA	3146568..3147218 (-)	651	WP_014618045.1	maleylacetoacetate isomerase	-
RSPO_RS14510 (RSPO_c03021)	-	3147222..3147923 (-)	702	WP_014618046.1	fumarylacetoacetate hydrolase family protein	-
RSPO_RS14515 (RSPO_c03022)	-	3148074..3148700 (+)	627	WP_014618047.1	ANTAR domain-containing response regulator	-
RSPO_RS14520 (RSPO_c03023)	-	3148979..3150241 (+)	1263	WP_014618048.1	CmpA/NrtA family ABC transporter substrate-binding protein	-
RSPO_RS14525 (RSPO_c03024)	ntrB	3150291..3151142 (+)	852	WP_042549711.1	nitrate ABC transporter permease	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 40168.48 Da
• Isoelectric Point: 5.8101

>NTDB_id=48880 RSPO_RS14500 WP_014618044.1 3145229..3146362(+) (pilA) [Ralstonia solanacearum Po82]
MFSFWKKRKAEPQPAAEPAPPAAAPEAVQAPVPAPVASPTPAPAPAPVAVPDTPAAPAALDMQADDIETVPTPPVMEQAR
KGWMSRLRSGLSKTSKNLTTLFVGVKVDEALFEELETALLMADAGVDATEYLLDELRRRVKAQRIETAESVKTALRDLLV
ELLHPLEKTMVLGRDQPMVIMIAGVNGAGKTTSIGKLCKHFQTYGQSVLLAAGDTFRAAAREQLVIWGQRNNVTVVAQES
GDPAAVIFDAVNAARARGIDIVMADTAGRLPTQMHLMEELKKVRRVIGKAMVTAPHETLLVIDANTGQNALAQVKAFDDA
LGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEQVEDLQPFSAREFADALLG

Nucleotide

• Length: 1134 bp

>NTDB_id=48880 RSPO_RS14500 WP_014618044.1 3145229..3146362(+) (pilA) [Ralstonia solanacearum Po82]
ATGTTTAGTTTCTGGAAGAAGCGCAAGGCAGAGCCCCAGCCGGCCGCCGAACCCGCGCCGCCCGCCGCCGCGCCCGAAGC
CGTCCAGGCCCCGGTACCGGCCCCGGTTGCCTCCCCCACCCCTGCCCCTGCACCCGCCCCGGTTGCCGTGCCGGATACGC
CGGCCGCGCCGGCTGCGCTGGACATGCAGGCCGACGACATCGAGACCGTACCGACGCCGCCCGTGATGGAGCAGGCGCGC
AAGGGCTGGATGTCTCGCCTGCGCTCGGGGCTGTCGAAGACCAGCAAGAACCTGACCACGCTGTTCGTCGGCGTGAAGGT
GGACGAGGCGCTGTTCGAGGAGCTGGAGACCGCGCTGCTGATGGCCGATGCCGGCGTGGATGCCACCGAATACCTGCTGG
ACGAGCTGCGCCGCCGCGTGAAGGCCCAGCGCATCGAGACCGCCGAGAGCGTGAAGACCGCGCTGCGCGACCTGCTCGTC
GAGCTGCTGCACCCGCTGGAAAAGACCATGGTGCTCGGCCGCGACCAGCCGATGGTGATCATGATCGCGGGCGTCAACGG
TGCCGGCAAGACCACCAGCATCGGCAAGCTGTGCAAGCACTTCCAGACCTACGGGCAGTCCGTGCTGCTGGCCGCCGGCG
ACACGTTCCGCGCCGCCGCCCGCGAGCAACTGGTGATCTGGGGCCAGCGCAACAACGTGACCGTGGTGGCGCAGGAGTCG
GGCGACCCGGCCGCCGTGATCTTCGACGCGGTGAACGCGGCGCGTGCCCGCGGCATCGACATTGTCATGGCCGACACCGC
CGGCCGCCTGCCGACCCAGATGCACCTGATGGAAGAGCTGAAGAAGGTGCGCCGCGTGATCGGCAAGGCCATGGTGACGG
CGCCGCACGAGACCCTGCTGGTGATCGACGCCAACACCGGCCAGAACGCGCTGGCGCAGGTGAAGGCTTTCGACGATGCA
CTCGGCCTGACCGGCCTGATCGTCACTAAGCTGGACGGCACCGCCAAAGGTGGCATCCTGGCCGCCATCGCGCGGCAGCG
GCCGGTGCCGGTGTATTTCATCGGCGTGGGCGAACAGGTCGAAGACCTGCAACCGTTCTCCGCGCGCGAATTCGCCGACG
CGCTGCTGGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (177-377)

• SRP54-type protein, helical bundle domain (SRP54_N) (96-157)

3D structure

• AlphaFold DB: F6G4B5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	51.202	100	0.565