Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RSPO_RS13565 Genome accession   NC_017574
Coordinates   2942211..2942717 (-) Length   168 a.a.
NCBI ID   WP_014617872.1    Uniprot ID   F6G3Z0
Organism   Ralstonia solanacearum Po82     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2921317..2943506 2942211..2942717 within 0


Gene organization within MGE regions


Location: 2921317..2943506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSPO_RS13480 (RSPO_c02804) - 2921317..2922078 (+) 762 WP_014617860.1 tyrosine-type recombinase/integrase -
  RSPO_RS13490 (RSPO_c02806) - 2922126..2923336 (+) 1211 WP_086002887.1 IS3 family transposase -
  RSPO_RS24145 (RSPO_c02807) - 2923351..2924073 (+) 723 WP_086002895.1 HNH endonuclease -
  RSPO_RS13505 (RSPO_c02809) - 2924198..2925321 (+) 1124 WP_086002891.1 IS3 family transposase -
  RSPO_RS25200 - 2925391..2925969 (+) 579 WP_124994724.1 hypothetical protein -
  RSPO_RS13510 (RSPO_c02810) - 2926132..2931123 (+) 4992 WP_014617862.1 DEAD/DEAH box helicase -
  RSPO_RS24150 (RSPO_c02811) - 2931740..2932942 (-) 1203 WP_014617863.1 helix-turn-helix domain-containing protein -
  RSPO_RS25205 (RSPO_c02812) - 2932945..2933607 (-) 663 WP_014617864.1 hypothetical protein -
  RSPO_RS13525 (RSPO_c02813) - 2933861..2934427 (+) 567 WP_042567706.1 recombinase family protein -
  RSPO_RS13530 (RSPO_c02814) - 2934481..2935245 (+) 765 WP_042567705.1 hypothetical protein -
  RSPO_RS25210 (RSPO_c02815) - 2935357..2936040 (+) 684 WP_124994723.1 hypothetical protein -
  RSPO_RS23645 (RSPO_c02816) - 2936040..2937722 (+) 1683 WP_014617868.1 AAA family ATPase -
  RSPO_RS25215 (RSPO_c02819) - 2939008..2939247 (-) 240 WP_014617869.1 hypothetical protein -
  RSPO_RS13555 (RSPO_c02820) - 2939384..2939830 (-) 447 WP_230626093.1 hypothetical protein -
  RSPO_RS24950 moaC 2940212..2940313 (+) 102 Protein_2705 cyclic pyranopterin monophosphate synthase MoaC -
  RSPO_RS13560 (RSPO_c02821) - 2940390..2942126 (-) 1737 WP_014617871.1 PglL family O-oligosaccharyltransferase -
  RSPO_RS13565 (RSPO_c02822) pilA 2942211..2942717 (-) 507 WP_014617872.1 pilin Machinery gene
  RSPO_RS13570 (RSPO_c02823) pilA 2942985..2943506 (-) 522 WP_014617873.1 pilin Machinery gene

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 16882.51 Da        Isoelectric Point: 9.9749

>NTDB_id=48874 RSPO_RS13565 WP_014617872.1 2942211..2942717(-) (pilA) [Ralstonia solanacearum Po82]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKSLVAENAANAQSDLSVGSSTFA
ATKNVSGLAINATAAPAGQITITYTTAAGNGTLALVPTSGGNALVVSSAPGAAIMWTCYSAGKATAASSVAASPAATLLP
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=48874 RSPO_RS13565 WP_014617872.1 2942211..2942717(-) (pilA) [Ralstonia solanacearum Po82]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACCATTCGTGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCGCAGGCCAAGTCGCTGGTGGCTGAGAATGCTGCAAACGCTCAGAGCGACCTGTCTGTGGGCTCGTCGACGTTTGCT
GCTACCAAGAACGTGTCCGGCCTTGCTATTAACGCGACTGCGGCACCGGCCGGCCAAATCACGATTACGTACACCACTGC
AGCGGGTAACGGTACGCTTGCCCTTGTCCCGACTTCGGGCGGTAATGCTTTGGTCGTGAGTTCCGCTCCGGGCGCAGCTA
TCATGTGGACCTGCTATTCTGCTGGTAAGGCCACGGCTGCCAGCAGCGTTGCTGCGAGCCCCGCGGCGACCCTGCTGCCG
AAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(44-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6G3Z0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

79.167

100

0.792
  pilA2 Legionella pneumophila str. Paris

52.174

95.833

0.5
  pilA2 Legionella pneumophila strain ERS1305867

52.174

95.833

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.265

100

0.446
  comP Acinetobacter baylyi ADP1

42.353

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.095

100

0.363

Multiple sequence alignment