Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RSPO_RS03250 Genome accession   NC_017574
Coordinates   713748..715013 (-) Length   421 a.a.
NCBI ID   WP_014616068.1    Uniprot ID   F6FY27
Organism   Ralstonia solanacearum Po82     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708748..720013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSPO_RS03220 (RSPO_c00676) - 709813..710697 (+) 885 WP_014616064.1 ATP-binding protein -
  RSPO_RS03225 (RSPO_c00677) - 710675..711136 (+) 462 WP_014616065.1 NUDIX domain-containing protein -
  RSPO_RS03230 (RSPO_c00678) - 711129..711344 (-) 216 WP_003266032.1 DNA gyrase inhibitor YacG -
  RSPO_RS03235 (RSPO_c00679) zapD 711361..712119 (-) 759 WP_003266031.1 cell division protein ZapD -
  RSPO_RS03240 (RSPO_c00680) coaE 712237..712851 (-) 615 WP_014616066.1 dephospho-CoA kinase -
  RSPO_RS03245 (RSPO_c00681) pilD 712864..713745 (-) 882 WP_014616067.1 prepilin peptidase Machinery gene
  RSPO_RS03250 (RSPO_c00682) pilG 713748..715013 (-) 1266 WP_014616068.1 type II secretion system F family protein Machinery gene
  RSPO_RS03255 (RSPO_c00683) pilF 715058..716785 (-) 1728 WP_014616069.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RSPO_RS03260 (RSPO_c00684) - 717033..718310 (-) 1278 WP_014616070.1 HlyC/CorC family transporter -
  RSPO_RS03270 (RSPO_c00685) ispB 718585..719553 (-) 969 WP_003271627.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46498.72 Da        Isoelectric Point: 10.4590

>NTDB_id=48863 RSPO_RS03250 WP_014616068.1 713748..715013(-) (pilG) [Ralstonia solanacearum Po82]
MATRAPTANRSAAASARKTASTKAPTQYIFEWEGKDRKGKAFKGEMRAESITEVNAVLRKQGLSITKSKRRRAARGKKIT
PKDVAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMNISDFFVKW
WYIIIFAPVVGIFLYARAFKRSLKVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YHDATLEIQQSVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=48863 RSPO_RS03250 WP_014616068.1 713748..715013(-) (pilG) [Ralstonia solanacearum Po82]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAAACCGCCTCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAAGCCTTCAAGGGCGAGATGCGGGCGGAGAGCATCACCGAAG
TCAACGCGGTGCTGCGCAAGCAGGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGCGCGGCGCGCGGCAAGAAGATCACG
CCCAAGGACGTGGCCTACTTCACGCGCCAGTTGTCGACCATGCTGAAGGCCGGGGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGCCACGCCAACCCGAACTTCACCCAGTTGCTGACGGAAATCCGCGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCTTTCCGCCGCCATCCGAAGTACTTCGATTCGCTGTACTGCAACCTGATCGACGCGGGCGAACAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTACCCAATCTCGGTGCTGACGGTGGCCTTCCTGGTCACGGTGGTGCTGATGATCTTCGTAGTGCCGGCGTTCAAGA
ACGTGTTCGCCAGCTTTGGCGCCACGCTGCCGGCACCGACGCTGGTGGTGATGAATATCTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTCGCCCCCGTCGTCGGCATCTTCCTGTATGCGCGGGCCTTCAAGCGCTCGCTGAAGGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGCA
CGCTGGCGACGATGTTCTCCGCCGGTACGCCGCTGGTGGAATCGATGGAGTCGGTGGCCGGCGCGGCCGGCAACTGGGTC
TACCATGACGCCACCCTCGAGATCCAGCAATCGGTGCGCATCGGTACCAGCCTCACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACACAGATCGGCGAGGAATCCGGTGCGCTGGACAACATGCTGCTGAAGGTGGCCG
AGTTCTACGAGCGCGAGGTCGATGACGCGGTGGCCAACATCTCCGCCCTGATCGAGCCGATCATCATCGTGATCCTCGGC
GTGCTGATCGGCGGCATGGTGGTCGCCATGTACCTGCCGATTTTCAAGCTGGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6FY27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.128

94.774

0.485
  pilG Neisseria meningitidis 44/76-A

50.877

94.774

0.482
  pilC Pseudomonas stutzeri DSM 10701

49.751

95.487

0.475
  pilC Acinetobacter baumannii D1279779

47.42

96.675

0.458
  pilC Legionella pneumophila strain ERS1305867

47.03

95.962

0.451
  pilC Acinetobacter baylyi ADP1

46.437

96.675

0.449
  pilC Vibrio campbellii strain DS40M4

38.537

97.387

0.375
  pilC Vibrio cholerae strain A1552

39.25

95.012

0.373

Multiple sequence alignment