Detailed information    

experimental Experimentally validated

Overview

Name   fimT   Type   Machinery gene
Locus tag   ACE17W_RS10880 Genome accession   NZ_CP169562
Coordinates   2414901..2415608 (-) Length   235 a.a.
NCBI ID   WP_010946369.1    Uniprot ID   Q5ZXU9
Organism   Legionella pneumophila strain Lp02     
Function   DNA uptake   
DNA binding and uptake

Function

FimT plays an important role in DNA uptake


Genomic Context

Location: 2409901..2420608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE17W_RS10865 (ACE17W_10865) - 2412776..2413288 (-) 513 WP_010946366.1 pilus assembly PilX family protein -
  ACE17W_RS10870 (ACE17W_10870) - 2413285..2414352 (-) 1068 WP_010946367.1 PilW family protein -
  ACE17W_RS10875 (ACE17W_10875) pilV 2414349..2414888 (-) 540 WP_010946368.1 type IV pilus modification protein PilV -
  ACE17W_RS10880 (ACE17W_10880) fimT 2414901..2415608 (-) 708 WP_010946369.1 GspH/FimT family pseudopilin Machinery gene
  ACE17W_RS10885 (ACE17W_10885) - 2415830..2416690 (-) 861 WP_011213225.1 polysaccharide deacetylase family protein -
  ACE17W_RS10890 (ACE17W_10890) - 2416867..2418216 (-) 1350 WP_010946371.1 lpg0634 family Dot/Icm T4SS effector -
  ACE17W_RS10895 (ACE17W_10895) - 2418504..2419985 (+) 1482 WP_010946372.1 phospholipid carrier-dependent glycosyltransferase -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 24868.47 Da        Isoelectric Point: 7.0384

>NTDB_id=1282 ACE17W_RS10880 WP_010946369.1 2414901..2415608(-) (fimT) [Legionella pneumophila strain Lp02]
MNKVITESQKFTLRNIANMLFCVESSVLPAQSNIAQKIQTQGIMYNDLCKDSFDTEAPLNSNPLSIAGFTLVELLVTLSV
FAIILTLIVPSLRTMILNSRLTSNIDSLVSSLNYARGVALDRAVNVAVCPLGSPGSTACGANWSSGWIVVTQPVAGAPTL
LKSHQTSVNDPVITSNVSSVVFDPHGLSTTQSNFTMCDNRGNAFARSAMVLATGFVQSGTTPGQAVWNNGALNCP

Nucleotide


Download         Length: 708 bp        

>NTDB_id=1282 ACE17W_RS10880 WP_010946369.1 2414901..2415608(-) (fimT) [Legionella pneumophila strain Lp02]
TTGAACAAAGTGATAACGGAATCACAAAAATTCACTCTGCGCAACATAGCTAATATGTTATTTTGCGTTGAGAGTAGTGT
GTTGCCCGCACAATCTAATATAGCACAAAAGATACAAACACAAGGCATAATGTACAATGATCTTTGTAAAGACAGCTTCG
ATACTGAGGCCCCTCTTAACAGCAACCCTCTTTCCATAGCCGGATTCACACTGGTTGAATTATTAGTAACCCTATCTGTT
TTTGCCATCATCTTAACTCTAATCGTCCCTTCATTAAGAACTATGATTTTGAATAGCCGTTTGACTTCAAACATTGACTC
ACTGGTAAGCTCATTAAACTATGCGCGTGGAGTGGCACTTGATCGGGCAGTCAATGTGGCAGTATGCCCATTAGGTTCAC
CAGGTTCAACGGCCTGTGGAGCCAACTGGAGCTCAGGATGGATAGTAGTGACCCAACCAGTAGCAGGAGCACCAACGCTT
TTAAAATCGCACCAGACTTCAGTGAATGATCCTGTTATCACTTCGAACGTATCCAGTGTTGTTTTTGACCCACATGGGTT
ATCAACAACCCAAAGTAACTTCACTATGTGTGATAACAGAGGCAATGCGTTTGCCCGCTCGGCAATGGTTTTGGCCACTG
GATTTGTCCAATCCGGAACAACACCCGGCCAAGCAGTCTGGAATAATGGAGCTTTGAACTGCCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(67-88)

Type II transport protein GspH (GspH)

(106-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZXU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Sebastian A G Braus et al. (2022) The molecular basis of FimT-mediated DNA uptake during bacterial natural transformation. Nature Communications 13(1):1065. [PMID: 35246533]