Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU68_RS01910 Genome accession   NZ_CP073946
Coordinates   419340..420017 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain MB51     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414340..425017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU68_RS01895 (KFU68_01855) chiA 415690..418400 (+) 2711 Protein_378 bifunctional chitinase/lysozyme -
  KFU68_RS01900 (KFU68_01860) bfd 418569..418763 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU68_RS01905 (KFU68_01865) bfr 418835..419311 (+) 477 WP_000675504.1 bacterioferritin -
  KFU68_RS01910 (KFU68_01870) pilD 419340..420017 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KFU68_RS01915 (KFU68_01875) gspM 420017..420478 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU68_RS01920 (KFU68_01880) gspL 420475..421638 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  KFU68_RS01925 (KFU68_01885) gspK 421653..422636 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU68_RS01930 (KFU68_01890) gspJ 422629..423216 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU68_RS01935 (KFU68_01895) gspI 423209..423586 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU68_RS01940 (KFU68_01900) gspH 423583..424092 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  KFU68_RS01945 (KFU68_01905) gspG 424100..424537 (-) 438 WP_257201933.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=488573 KFU68_RS01910 WP_000178161.1 419340..420017(-) (pilD) [Escherichia coli strain MB51]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=488573 KFU68_RS01910 WP_000178161.1 419340..420017(-) (pilD) [Escherichia coli strain MB51]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378