Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   KFU89_RS18575 Genome accession   NZ_CP073943
Coordinates   3869970..3871352 (-) Length   460 a.a.
NCBI ID   WP_222896338.1    Uniprot ID   -
Organism   Escherichia coli strain MB52     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3864970..3876352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU89_RS18565 (KFU89_18450) ettA 3866743..3868410 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  KFU89_RS18570 (KFU89_18455) nadR 3868717..3869949 (-) 1233 WP_000093813.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  KFU89_RS18575 (KFU89_18460) radA/sms 3869970..3871352 (-) 1383 WP_222896338.1 DNA repair protein RadA Machinery gene
  KFU89_RS18580 (KFU89_18465) serB 3871401..3872369 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  KFU89_RS18585 (KFU89_18470) ytjB 3872475..3873119 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  KFU89_RS18590 (KFU89_18475) lplA 3873147..3874163 (+) 1017 WP_000105890.1 lipoate--protein ligase LplA -
  KFU89_RS18595 (KFU89_18480) - 3874195..3874458 (+) 264 WP_000566155.1 helix-turn-helix domain-containing protein -
  KFU89_RS18600 (KFU89_18485) deoD 3874619..3875338 (-) 720 WP_000224879.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49470.07 Da        Isoelectric Point: 7.4219

>NTDB_id=488545 KFU89_RS18575 WP_222896338.1 3869970..3871352(-) (radA/sms) [Escherichia coli strain MB52]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPRDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=488545 KFU89_RS18575 WP_222896338.1 3869970..3871352(-) (radA/sms) [Escherichia coli strain MB52]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGTGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGACATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTATTAGGCGGCGGCGTGGTACCGGGAAGTGCCATTCTGATTGGCGGTAACCCAGGCGCGGG
GAAATCCACGCTGTTGCTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAG
CAGATCTGCCTGATTGCTGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCCATCCAGGTGATGCATATGGCGGATGT
ACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAG
TGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCGGTAGGTCTGGAGCAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTATTTGTGAACGTGGTCGGTGGTGTAAAGGTAACCGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTACCGCGGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAGCGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.297

98.913

0.428
  radA Streptococcus pneumoniae D39

42.857

98.913

0.424
  radA Streptococcus pneumoniae TIGR4

42.857

98.913

0.424
  radA Streptococcus mitis NCTC 12261

42.857

98.913

0.424
  radA Streptococcus pneumoniae R6

42.857

98.913

0.424
  radA Streptococcus pneumoniae Rx1

42.857

98.913

0.424