Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU83_RS02035 Genome accession   NZ_CP073939
Coordinates   429684..430361 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain MB53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 424684..435361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU83_RS02010 (KFU83_01970) - 424849..425523 (-) 675 Protein_402 lysozyme -
  KFU83_RS02015 (KFU83_01975) - 425575..426783 (+) 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
  KFU83_RS02020 (KFU83_01980) chiA 426795..428744 (+) 1950 Protein_404 bifunctional chitinase/lysozyme -
  KFU83_RS02025 (KFU83_01985) bfd 428913..429107 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU83_RS02030 (KFU83_01990) bfr 429179..429655 (+) 477 WP_000675504.1 bacterioferritin -
  KFU83_RS02035 (KFU83_01995) pilD 429684..430361 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KFU83_RS02040 (KFU83_02000) gspM 430361..430822 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU83_RS02045 (KFU83_02005) gspL 430819..431982 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KFU83_RS02050 (KFU83_02010) gspK 431997..432980 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU83_RS02055 (KFU83_02015) gspJ 432973..433560 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU83_RS02060 (KFU83_02020) gspI 433553..433930 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU83_RS02065 (KFU83_02025) gspH 433927..434436 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  KFU83_RS02070 (KFU83_02030) gspG 434444..434881 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=488416 KFU83_RS02035 WP_000178161.1 429684..430361(-) (pilD) [Escherichia coli strain MB53]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=488416 KFU83_RS02035 WP_000178161.1 429684..430361(-) (pilD) [Escherichia coli strain MB53]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378