Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU91_RS02060 Genome accession   NZ_CP073929
Coordinates   447974..448651 (-) Length   225 a.a.
NCBI ID   WP_025670758.1    Uniprot ID   -
Organism   Escherichia coli strain MB64     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442974..453651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU91_RS02045 (KFU91_02000) chiA 444341..447034 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KFU91_RS02050 (KFU91_02005) bfd 447203..447397 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU91_RS02055 (KFU91_02010) bfr 447469..447945 (+) 477 WP_000675504.1 bacterioferritin -
  KFU91_RS02060 (KFU91_02015) pilD 447974..448651 (-) 678 WP_025670758.1 prepilin peptidase GspO Machinery gene
  KFU91_RS02065 (KFU91_02020) gspM 448651..449112 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU91_RS02070 (KFU91_02025) gspL 449109..450272 (-) 1164 WP_257198866.1 type II secretion system protein GspL -
  KFU91_RS02075 (KFU91_02030) gspK 450287..451270 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU91_RS02080 (KFU91_02035) gspJ 451263..451850 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU91_RS02085 (KFU91_02040) gspI 451843..452220 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU91_RS02090 (KFU91_02045) gspH 452217..452726 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  KFU91_RS02095 (KFU91_02050) gspG 452734..453171 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24888.75 Da        Isoelectric Point: 7.9563

>NTDB_id=488172 KFU91_RS02060 WP_025670758.1 447974..448651(-) (pilD) [Escherichia coli strain MB64]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLSDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=488172 KFU91_RS02060 WP_025670758.1 447974..448651(-) (pilD) [Escherichia coli strain MB64]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGTCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378