Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU77_RS02225 Genome accession   NZ_CP073926
Coordinates   477727..478404 (-) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain MB98     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 472727..483404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU77_RS02210 (KFU77_02185) chiA 474094..476787 (+) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  KFU77_RS02215 (KFU77_02190) bfd 476956..477150 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KFU77_RS02220 (KFU77_02195) bfr 477222..477698 (+) 477 WP_000675504.1 bacterioferritin -
  KFU77_RS02225 (KFU77_02200) pilD 477727..478404 (-) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  KFU77_RS02230 (KFU77_02205) gspM 478404..478865 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KFU77_RS02235 (KFU77_02210) gspL 478862..480025 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  KFU77_RS02240 (KFU77_02215) gspK 480040..481023 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KFU77_RS02245 (KFU77_02220) gspJ 481016..481603 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KFU77_RS02250 (KFU77_02225) gspI 481596..481973 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KFU77_RS02255 (KFU77_02230) gspH 481970..482479 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  KFU77_RS02260 (KFU77_02235) gspG 482487..482924 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=488090 KFU77_RS02225 WP_001377644.1 477727..478404(-) (pilD) [Escherichia coli strain MB98]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=488090 KFU77_RS02225 WP_001377644.1 477727..478404(-) (pilD) [Escherichia coli strain MB98]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373