Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   KD735_RS20310 Genome accession   NZ_CP073624
Coordinates   4154296..4155678 (-) Length   460 a.a.
NCBI ID   WP_001029694.1    Uniprot ID   -
Organism   Escherichia coli strain AW1.7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4149296..4160678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KD735_RS20295 (KD735_20115) ettA 4151068..4152735 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  KD735_RS20300 (KD735_20120) nadS 4152896..4153009 (-) 114 Protein_3985 NadS family protein -
  KD735_RS20305 (KD735_20125) nadR 4153043..4154275 (-) 1233 WP_000093812.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  KD735_RS20310 (KD735_20130) radA/sms 4154296..4155678 (-) 1383 WP_001029694.1 DNA repair protein RadA Machinery gene
  KD735_RS20315 (KD735_20135) serB 4155727..4156695 (-) 969 WP_001132956.1 phosphoserine phosphatase -
  KD735_RS20320 (KD735_20140) ytjB 4156801..4157445 (+) 645 WP_000124615.1 YtjB family periplasmic protein -
  KD735_RS20325 (KD735_20145) lplA 4157473..4158489 (+) 1017 WP_054626345.1 lipoate--protein ligase LplA -
  KD735_RS20330 - 4158521..4158784 (+) 264 Protein_3991 helix-turn-helix domain-containing protein -
  KD735_RS20335 (KD735_20155) deoD 4158945..4159664 (-) 720 WP_000224877.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49530.14 Da        Isoelectric Point: 6.9933

>NTDB_id=487538 KD735_RS20310 WP_001029694.1 4154296..4155678(-) (radA/sms) [Escherichia coli strain AW1.7]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKMRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKEPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=487538 KD735_RS20310 WP_001029694.1 4154296..4155678(-) (radA/sms) [Escherichia coli strain AW1.7]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGGGTGGTGCCGGGAAGCGCCATTCTGATTGGCGGTAACCCAGGTGCCGG
GAAATCCACATTGCTATTGCAAACGCTGTGCAAACTGGCGCAGCAGATGAAAACGCTGTATGTCACTGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTATCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAATCATCGCCAGGCAGCGTGGCGCAGGTGCGTGAAACAGCGGCTTATCTGACGCGCTTCGCCAAAATGCGCGGTGTGG
CGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAG
TGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCG
CCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGAGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.608

99.348

0.463
  radA Streptococcus mitis NCTC 12261

43.64

99.13

0.433
  radA Streptococcus mitis SK321

43.64

99.13

0.433
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43