Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KD735_RS02310 Genome accession   NZ_CP073624
Coordinates   506266..506943 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain AW1.7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501266..511943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KD735_RS02295 (KD735_02260) chiA 502633..505326 (+) 2694 WP_000773174.1 bifunctional chitinase/lysozyme -
  KD735_RS02300 (KD735_02265) bfd 505495..505689 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KD735_RS02305 (KD735_02270) bfr 505761..506237 (+) 477 WP_000675504.1 bacterioferritin -
  KD735_RS02310 (KD735_02275) pilD 506266..506943 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  KD735_RS02315 (KD735_02280) gspM 506943..507404 (-) 462 WP_001549764.1 type II secretion system protein GspM -
  KD735_RS02320 (KD735_02285) gspL 507401..508564 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  KD735_RS02325 (KD735_02290) gspK 508579..509562 (-) 984 Protein_461 type II secretion system minor pseudopilin GspK -
  KD735_RS02330 (KD735_02295) gspJ 509555..510142 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KD735_RS02335 (KD735_02300) gspI 510135..510512 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KD735_RS02340 (KD735_02305) gspH 510509..511018 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  KD735_RS02345 (KD735_02310) gspG 511026..511463 (-) 438 WP_054626323.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=487486 KD735_RS02310 WP_000178161.1 506266..506943(-) (pilD) [Escherichia coli strain AW1.7]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=487486 KD735_RS02310 WP_000178161.1 506266..506943(-) (pilD) [Escherichia coli strain AW1.7]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378