Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   KCG56_RS07300 Genome accession   NZ_CP073116
Coordinates   1529864..1530343 (+) Length   159 a.a.
NCBI ID   WP_283255477.1    Uniprot ID   -
Organism   Neisseria subflava strain TT0073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1524864..1535343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG56_RS07280 (KCG56_07355) - 1525319..1525759 (+) 441 WP_254321026.1 TIGR01244 family sulfur transferase -
  KCG56_RS07285 (KCG56_07360) - 1526199..1526495 (+) 297 WP_254321027.1 helix-turn-helix transcriptional regulator -
  KCG56_RS07290 (KCG56_07365) glnD 1526515..1529072 (+) 2558 Protein_1414 [protein-PII] uridylyltransferase -
  KCG56_RS07300 (KCG56_07370) pilA2 1529864..1530343 (+) 480 WP_283255477.1 pilin Machinery gene
  KCG56_RS11610 (KCG56_07375) pilA/pilA1 1530450..1530923 (+) 474 WP_283255478.1 pilin Machinery gene
  KCG56_RS07315 (KCG56_07380) - 1531328..1531810 (+) 483 WP_003684427.1 Lrp/AsnC family transcriptional regulator -
  KCG56_RS07320 (KCG56_07385) - 1531928..1532524 (+) 597 WP_254321028.1 DUF1415 domain-containing protein -
  KCG56_RS07325 (KCG56_07390) - 1532539..1532898 (+) 360 WP_254321029.1 YbaN family protein -
  KCG56_RS07330 (KCG56_07395) - 1533341..1534758 (+) 1418 Protein_1420 sodium:proton antiporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16602.26 Da        Isoelectric Point: 8.4616

>NTDB_id=486928 KCG56_RS07300 WP_283255477.1 1529864..1530343(+) (pilA2) [Neisseria subflava strain TT0073]
MKAIQKGFTLIELMIVIAILGILAVIALPAYQDYTVRTKVSEGLGLAAPAKLAVVETSAALGGLKKVTAANSGYKFVPTK
YVQSIEIKEDGVIKVVTKDTGAKIQPAFTLTPSQASDNIEAPIEWACTKDAGEEKHLPANCRTATTPASTTATPAASTN

Nucleotide


Download         Length: 480 bp        

>NTDB_id=486928 KCG56_RS07300 WP_283255477.1 1529864..1530343(+) (pilA2) [Neisseria subflava strain TT0073]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAATTGATGATCGTCATCGCCATCTTGGGCATCTTGGCCGTAATCGC
TCTGCCTGCATACCAAGACTACACTGTCCGCACTAAAGTGTCTGAAGGCTTAGGCCTCGCAGCTCCTGCAAAATTAGCTG
TGGTTGAAACTTCCGCAGCGCTTGGTGGATTGAAGAAAGTTACTGCTGCTAATAGTGGTTATAAATTTGTACCTACTAAG
TACGTTCAAAGTATTGAAATTAAAGAAGACGGTGTAATTAAAGTTGTAACTAAAGATACTGGTGCTAAAATACAACCAGC
ATTTACGTTAACTCCAAGCCAAGCTTCAGATAATATTGAAGCACCAATCGAGTGGGCATGTACTAAAGATGCTGGTGAAG
AGAAACATTTGCCTGCTAACTGCCGAACTGCTACTACTCCTGCTTCTACTACTGCTACGCCTGCTGCTTCTACTAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

48.201

87.421

0.421
  pilA2 Legionella pneumophila str. Paris

47.482

87.421

0.415
  pilE Neisseria gonorrhoeae MS11

38.462

100

0.409
  pilA Ralstonia pseudosolanacearum GMI1000

39.634

100

0.409
  pilE Neisseria gonorrhoeae strain FA1090

37.952

100

0.396
  comP Acinetobacter baylyi ADP1

41.611

93.711

0.39
  pilA/pilA1 Eikenella corrodens VA1

38.125

100

0.384
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.816

92.453

0.377
  pilA Glaesserella parasuis strain SC1401

37.821

98.113

0.371