Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IHE28_RS10570 Genome accession   NZ_CP062178
Coordinates   2341726..2343249 (+) Length   507 a.a.
NCBI ID   WP_104076582.1    Uniprot ID   A0A2P5KDW7
Organism   Mycetohabitans endofungorum strain B5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2336726..2348249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE28_RS10530 (IHE28_10445) - 2336861..2336995 (-) 135 Protein_2048 LysE family translocator -
  IHE28_RS10535 (IHE28_10450) - 2337029..2337595 (-) 567 WP_104076633.1 adenine phosphoribosyltransferase -
  IHE28_RS10540 - 2337675..2337806 (-) 132 WP_255779347.1 hypothetical protein -
  IHE28_RS10545 (IHE28_10455) - 2337916..2338899 (+) 984 WP_104076586.1 KpsF/GutQ family sugar-phosphate isomerase -
  IHE28_RS10550 (IHE28_10460) - 2338907..2339437 (+) 531 WP_104076585.1 HAD family hydrolase -
  IHE28_RS10555 (IHE28_10465) lptC 2339441..2340046 (+) 606 WP_104076584.1 LPS export ABC transporter periplasmic protein LptC -
  IHE28_RS10560 (IHE28_10470) lptA 2340083..2340778 (+) 696 WP_104076632.1 lipopolysaccharide transport periplasmic protein LptA -
  IHE28_RS10565 (IHE28_10475) lptB 2340775..2341554 (+) 780 WP_104076583.1 LPS export ABC transporter ATP-binding protein -
  IHE28_RS10570 (IHE28_10480) rpoN/rpoN1 2341726..2343249 (+) 1524 WP_104076582.1 RNA polymerase factor sigma-54 Machinery gene
  IHE28_RS10575 (IHE28_10485) raiA 2343437..2343787 (+) 351 WP_104076581.1 ribosome-associated translation inhibitor RaiA -
  IHE28_RS10580 (IHE28_10490) ptsN 2344042..2344497 (+) 456 WP_076796362.1 PTS IIA-like nitrogen regulatory protein PtsN -
  IHE28_RS10585 (IHE28_10495) hprK 2344595..2345563 (+) 969 WP_041754506.1 HPr(Ser) kinase/phosphatase -
  IHE28_RS10590 (IHE28_10500) rapZ 2345617..2346531 (+) 915 WP_233203074.1 RNase adapter RapZ -
  IHE28_RS10595 (IHE28_10505) - 2346557..2347195 (+) 639 WP_104076579.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 55343.69 Da        Isoelectric Point: 5.1625

>NTDB_id=486802 IHE28_RS10570 WP_104076582.1 2341726..2343249(+) (rpoN/rpoN1) [Mycetohabitans endofungorum strain B5]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENEDDWIASPLRVAADGSLLPSQSSALPTGMAE
APAPVLGSASGVNGERAESAEPAAVDEYNGMNSDSGTDTASWNLDDYVRPGGTSDDDDMPPLQLHESTVSLREHLGAQLR
LTKASSRDRALVTFLIESLDDDGYLSASCDEILADLPAELEVDADELNAALALLHSFDPAGVGARSASECLKLQLLRLEP
SATRTLALEIVSNYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKVEADYVAPDIIVKKTSQGWQAEL
NREVVPRLRVNHLYANILRSNRGDPGSGSLRQQLQEARWLIKNIQQRFDTILRVAQAIVERQRSFFVHGEIAMRPLVLRE
IADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQ
GFVIARRTVAKYREALKIPAVSMRKSL

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=486802 IHE28_RS10570 WP_104076582.1 2341726..2343249(+) (rpoN/rpoN1) [Mycetohabitans endofungorum strain B5]
ATGAAAGCAAGCCTTCAACTCCGTCTATCGCAGCATCTGGCACTGACCCCGCAATTGCAGCAGTCGATCCGGCTACTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAAGTCGCCACGGCGATCGCGCAAAATCCGTTACTGGAGAATGAGGACGACTGGA
TCGCGAGCCCATTGCGGGTCGCCGCGGACGGCTCACTGTTGCCGTCGCAAAGCAGCGCGCTACCCACCGGCATGGCCGAG
GCGCCAGCACCGGTGCTCGGCAGCGCCAGCGGCGTGAACGGCGAGCGCGCGGAAAGCGCGGAGCCGGCCGCCGTTGACGA
ATACAACGGCATGAACAGCGACAGCGGCACCGACACGGCGTCGTGGAACTTGGACGACTATGTGCGTCCCGGTGGCACCT
CCGACGACGACGACATGCCGCCGCTGCAGCTGCACGAATCCACGGTCAGCCTACGCGAGCATTTGGGCGCGCAGTTGCGC
CTGACCAAGGCTAGCTCGCGCGACCGCGCGCTGGTCACGTTTCTGATCGAGTCGCTGGACGACGATGGCTACCTGAGCGC
GAGCTGCGACGAAATCCTCGCCGACTTGCCGGCCGAGCTTGAGGTCGACGCCGATGAGTTGAACGCGGCGCTCGCGCTGC
TGCACAGCTTCGATCCGGCTGGGGTCGGGGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAATTGTTGCGGCTGGAGCCG
AGCGCCACGCGCACGCTGGCGCTGGAAATCGTGTCGAACTACCTGGAGTTGCTGGCGGCGCGCGACTTCACGCGTCTGCG
CAAGCATCTGAAGGCCAGCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCG
CGTACGGCAAGGTCGAGGCCGACTACGTGGCGCCGGACATCATCGTGAAGAAAACCTCACAGGGATGGCAGGCGGAGTTG
AACCGTGAAGTCGTGCCGCGGCTGCGGGTCAACCACTTGTACGCGAACATCCTGCGCAGCAACCGGGGTGACCCAGGCAG
CGGTTCGTTGCGCCAGCAACTGCAAGAAGCGCGCTGGCTGATCAAGAACATCCAGCAACGATTTGACACGATTTTGCGCG
TCGCACAAGCAATCGTCGAGCGTCAGCGCAGTTTTTTTGTGCATGGCGAGATCGCGATGCGCCCCTTGGTTTTACGAGAA
ATTGCTGATACGCTGGGCTTGCATGAGTCGACCGTATCGCGAGTCACGACCGGGAAGTACATGCTGACGCCCTTTGGGAC
GCTCGAGTTCAAGTACTTCTTTGGCTCGCACGTGTCGACCGATACGGGGGGCGCGGCGTCATCGACCGCGATCCGCGCGC
TCATCAAGCAACTGATAGGAGCCGAAGACCCGAAATCACCTCTTTCAGACAGCCGCATCGCTGAACTGCTGGCCGAACAG
GGCTTCGTGATCGCACGCCGAACTGTGGCGAAATATCGCGAAGCGCTGAAGATTCCGGCTGTTAGCATGCGTAAGTCGTT
ATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-334)

Sigma-54, DNA binding domain (Sigma54_DBD)

(350-505)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P5KDW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.451

100

0.679