Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IHE31_RS14345 Genome accession   NZ_CP062174
Coordinates   2399265..2400779 (+) Length   504 a.a.
NCBI ID   WP_338860279.1    Uniprot ID   -
Organism   Mycetohabitans rhizoxinica strain B47     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2394265..2405779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE31_RS14320 (IHE31_14005) - 2395462..2396445 (+) 984 WP_237070936.1 KpsF/GutQ family sugar-phosphate isomerase -
  IHE31_RS14325 (IHE31_14010) - 2396453..2396983 (+) 531 WP_013436347.1 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase -
  IHE31_RS14330 (IHE31_14015) lptC 2396987..2397592 (+) 606 WP_338860278.1 LPS export ABC transporter periplasmic protein LptC -
  IHE31_RS14335 (IHE31_14020) lptA 2397629..2398318 (+) 690 WP_237071024.1 lipopolysaccharide transport periplasmic protein LptA -
  IHE31_RS14340 (IHE31_14025) lptB 2398315..2399094 (+) 780 WP_338862317.1 LPS export ABC transporter ATP-binding protein -
  IHE31_RS14345 (IHE31_14030) rpoN/rpoN1 2399265..2400779 (+) 1515 WP_338860279.1 RNA polymerase factor sigma-54 Machinery gene
  IHE31_RS14350 (IHE31_14035) raiA 2400966..2401316 (+) 351 WP_041753863.1 ribosome-associated translation inhibitor RaiA -
  IHE31_RS14355 (IHE31_14040) ptsN 2401573..2402028 (+) 456 WP_013436353.1 PTS IIA-like nitrogen regulatory protein PtsN -
  IHE31_RS14360 (IHE31_14045) hprK 2402126..2403094 (+) 969 WP_041754506.1 HPr(Ser) kinase/phosphatase -
  IHE31_RS14365 (IHE31_14050) rapZ 2403149..2404063 (+) 915 WP_338863032.1 RNase adapter RapZ -
  IHE31_RS14370 (IHE31_14055) - 2404088..2404726 (+) 639 WP_013436356.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 55097.38 Da        Isoelectric Point: 5.0661

>NTDB_id=486777 IHE31_RS14345 WP_338860279.1 2399265..2400779(+) (rpoN/rpoN1) [Mycetohabitans rhizoxinica strain B47]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENENDWIASPLRVAADGSLVPSQSSALPTGMAE
APAPVPSGVNGERAQSVEPAAVDEYDGMSSDSGTDTASWNLDDYVRPGGTSDDDDMPPLQLHESTVSLREHLSTQLRLTQ
ASPRDRALVTFLIESLDDDGYLSASCDEILADLPAELEVDADELNAALALLHSFDPAGVGARSASECLKLQLLRLEPSAT
RTLALEIVSNYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTSQGWQAELNPE
VVPRLRVNHLYANILRSNRGDPSSGSLRQQLQEARWLIKNIQQRFDTILRVAQAIVERQRSFFAHGEIAMRPLVLREIAD
TLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFV
VARRTVAKYREALKIPAVSMRKSL

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=486777 IHE31_RS14345 WP_338860279.1 2399265..2400779(+) (rpoN/rpoN1) [Mycetohabitans rhizoxinica strain B47]
ATGAAAGCAAGCCTCCAACTCCGTCTATCACAGCATCTGGCGCTGACTCCGCAATTGCAGCAGTCGATCCGGCTACTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAGGTCGCCACGGCGATCGCACAGAATCCCTTGCTGGAGAACGAGAACGACTGGA
TCGCGAGCCCGTTGCGGGTCGCCGCGGACGGCTCACTGGTGCCGTCGCAAAGCAGCGCGCTGCCCACCGGCATGGCCGAG
GCGCCAGCACCGGTGCCCAGCGGCGTGAACGGCGAGCGCGCGCAAAGCGTGGAGCCGGCCGCCGTCGACGAATACGACGG
CATGAGCAGCGACAGCGGCACCGACACGGCGTCGTGGAACCTAGACGACTATGTGCGTCCCGGTGGCACCTCCGACGACG
ACGACATGCCGCCGCTGCAGCTGCACGAATCCACGGTCAGCCTGCGCGAGCATTTGAGCACGCAGTTGCGCCTGACCCAG
GCTAGCCCGCGCGACCGCGCGCTGGTCACGTTTTTGATCGAGTCGCTGGACGACGATGGCTACCTGAGCGCAAGTTGCGA
CGAAATCCTCGCCGATTTGCCGGCCGAGCTTGAGGTAGACGCCGATGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCT
TCGATCCGGCAGGAGTCGGGGCGCGCTCAGCGTCCGAATGCCTGAAGCTGCAATTGTTGCGGCTGGAGCCGAGCGCCACG
CGCACGCTGGCGCTGGAAATCGTGTCGAACTATCTAGAGTTGCTGGCGGCGCGCGACTTCACGCGTCTGCGCAAGCACCT
GAAGGCCAGCGACGACGCGCTGCGCGACGCGCACGCACTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCGCGTACGGCA
AGGCCGAGGCCGACTACGTGGTGCCGGACATCATCGTGAAGAAAACCTCGCAGGGATGGCAGGCGGAATTGAATCCCGAA
GTCGTGCCGCGGCTGCGGGTCAACCACTTATACGCGAACATCCTGCGCAGCAACCGAGGCGACCCGAGCAGCGGTTCGTT
GCGCCAGCAACTGCAGGAAGCGCGCTGGTTGATCAAGAACATCCAGCAACGTTTCGACACGATTTTGCGCGTCGCACAAG
CGATCGTCGAGCGTCAGCGCAGTTTTTTTGCGCATGGCGAGATCGCGATGCGCCCCTTGGTTTTACGAGAAATTGCTGAT
ACGCTGGGCTTGCATGAGTCGACCGTATCGCGGGTCACGACTGGGAAGTACATGTTGACGCCCTTTGGGACGCTCGAGTT
CAAGTACTTCTTTGGCTCGCATGTATCAACTGACACGGGGGGCGCGGCGTCATCGACCGCGATCCGCGCGCTTATCAAGC
AACTGATAGGAGCCGAAGACCCAAAATCACCTCTTTCAGATAGCCGGATCGCTGAACTGCTGGCCGAACAAGGCTTCGTG
GTCGCACGTCGAACTGTGGCGAAATATCGCGAAGCGCTGAAGATTCCAGCTGTTAGCATGCGTAAGTCGTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-331)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(347-502)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.442

100

0.688