Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IHE32_RS11180 Genome accession   NZ_CP062171
Coordinates   2456997..2458511 (+) Length   504 a.a.
NCBI ID   WP_338860279.1    Uniprot ID   -
Organism   Mycetohabitans rhizoxinica strain B49     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2451997..2463511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE32_RS11155 (IHE32_10965) - 2453194..2454177 (+) 984 WP_041753862.1 KpsF/GutQ family sugar-phosphate isomerase -
  IHE32_RS11160 (IHE32_10970) - 2454185..2454715 (+) 531 WP_013436347.1 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase -
  IHE32_RS11165 (IHE32_10975) lptC 2454719..2455324 (+) 606 WP_338860278.1 LPS export ABC transporter periplasmic protein LptC -
  IHE32_RS11170 (IHE32_10980) lptA 2455361..2456050 (+) 690 WP_049786487.1 lipopolysaccharide transport periplasmic protein LptA -
  IHE32_RS11175 (IHE32_10985) lptB 2456047..2456826 (+) 780 WP_013436350.1 LPS export ABC transporter ATP-binding protein -
  IHE32_RS11180 (IHE32_10990) rpoN/rpoN1 2456997..2458511 (+) 1515 WP_338860279.1 RNA polymerase factor sigma-54 Machinery gene
  IHE32_RS11185 (IHE32_10995) raiA 2458698..2459048 (+) 351 WP_041753863.1 ribosome-associated translation inhibitor RaiA -
  IHE32_RS11190 (IHE32_11000) ptsN 2459305..2459760 (+) 456 WP_013436353.1 PTS IIA-like nitrogen regulatory protein PtsN -
  IHE32_RS11195 (IHE32_11005) hprK 2459858..2460826 (+) 969 WP_041754506.1 HPr(Ser) kinase/phosphatase -
  IHE32_RS11200 (IHE32_11010) rapZ 2460881..2461795 (+) 915 WP_237071025.1 RNase adapter RapZ -
  IHE32_RS11205 (IHE32_11015) - 2461820..2462458 (+) 639 WP_013436356.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 55097.38 Da        Isoelectric Point: 5.0661

>NTDB_id=486764 IHE32_RS11180 WP_338860279.1 2456997..2458511(+) (rpoN/rpoN1) [Mycetohabitans rhizoxinica strain B49]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENENDWIASPLRVAADGSLVPSQSSALPTGMAE
APAPVPSGVNGERAQSVEPAAVDEYDGMSSDSGTDTASWNLDDYVRPGGTSDDDDMPPLQLHESTVSLREHLSTQLRLTQ
ASPRDRALVTFLIESLDDDGYLSASCDEILADLPAELEVDADELNAALALLHSFDPAGVGARSASECLKLQLLRLEPSAT
RTLALEIVSNYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTSQGWQAELNPE
VVPRLRVNHLYANILRSNRGDPSSGSLRQQLQEARWLIKNIQQRFDTILRVAQAIVERQRSFFAHGEIAMRPLVLREIAD
TLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFV
VARRTVAKYREALKIPAVSMRKSL

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=486764 IHE32_RS11180 WP_338860279.1 2456997..2458511(+) (rpoN/rpoN1) [Mycetohabitans rhizoxinica strain B49]
ATGAAAGCAAGCCTCCAACTCCGTCTATCACAGCATCTGGCGCTGACTCCGCAATTGCAGCAGTCGATCCGGCTACTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAGGTCGCCACGGCGATCGCACAGAATCCCTTGCTGGAGAACGAGAACGACTGGA
TCGCGAGCCCGTTGCGGGTCGCCGCGGACGGCTCACTGGTGCCGTCGCAAAGCAGCGCGCTGCCCACCGGCATGGCCGAG
GCGCCAGCACCGGTGCCCAGCGGCGTGAACGGCGAGCGCGCGCAAAGCGTGGAGCCGGCCGCCGTCGACGAATACGACGG
CATGAGCAGCGACAGCGGCACCGACACGGCGTCGTGGAACCTAGACGACTATGTGCGTCCCGGTGGCACCTCCGACGACG
ACGACATGCCGCCGCTGCAGCTGCACGAATCCACGGTCAGCCTGCGCGAGCATTTGAGCACGCAGTTGCGCCTGACCCAG
GCTAGCCCGCGCGACCGCGCGCTGGTCACGTTTTTGATCGAGTCGCTGGACGACGATGGCTACCTGAGCGCAAGTTGCGA
CGAAATCCTCGCCGATTTGCCGGCCGAGCTTGAGGTAGACGCCGATGAGCTGAACGCGGCGCTCGCGCTGCTGCACAGCT
TCGATCCGGCAGGAGTCGGGGCGCGCTCAGCGTCCGAATGCCTGAAGCTGCAATTGTTGCGGCTGGAGCCGAGCGCCACG
CGCACGCTGGCGCTGGAAATCGTGTCGAACTATCTAGAGTTGCTGGCGGCGCGCGACTTCACGCGTCTGCGCAAGCACCT
GAAGGCCAGCGACGACGCGCTGCGCGACGCGCACGCACTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCGCGTACGGCA
AGGCCGAGGCCGACTACGTGGTGCCGGACATCATCGTGAAGAAAACCTCGCAGGGATGGCAGGCGGAATTGAATCCCGAA
GTCGTGCCGCGGCTGCGGGTCAACCACTTATACGCGAACATCCTGCGCAGCAACCGAGGCGACCCGAGCAGCGGTTCGTT
GCGCCAGCAACTGCAGGAAGCGCGCTGGTTGATCAAGAACATCCAGCAACGTTTCGACACGATTTTGCGCGTCGCACAAG
CGATCGTCGAGCGTCAGCGCAGTTTTTTTGCGCATGGCGAGATCGCGATGCGCCCCTTGGTTTTACGAGAAATTGCTGAT
ACGCTGGGCTTGCATGAGTCGACCGTATCGCGGGTCACGACTGGGAAGTACATGTTGACGCCCTTTGGGACGCTCGAGTT
CAAGTACTTCTTTGGCTCGCATGTATCAACTGACACGGGGGGCGCGGCGTCATCGACCGCGATCCGCGCGCTTATCAAGC
AACTGATAGGAGCCGAAGACCCAAAATCACCTCTTTCAGATAGCCGGATCGCTGAACTGCTGGCCGAACAAGGCTTCGTG
GTCGCACGTCGAACTGTGGCGAAATATCGCGAAGCGCTGAAGATTCCAGCTGTTAGCATGCGTAAGTCGTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-331)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(347-502)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.442

100

0.688