Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IHE33_RS10860 Genome accession   NZ_CP062168
Coordinates   2464873..2466396 (+) Length   507 a.a.
NCBI ID   WP_338925859.1    Uniprot ID   -
Organism   Mycetohabitans endofungorum strain B60     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2459873..2471396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHE33_RS10835 (IHE33_10695) - 2461063..2462046 (+) 984 WP_338925855.1 KpsF/GutQ family sugar-phosphate isomerase -
  IHE33_RS10840 (IHE33_10700) - 2462054..2462584 (+) 531 WP_338925856.1 HAD family hydrolase -
  IHE33_RS10845 (IHE33_10705) lptC 2462588..2463193 (+) 606 WP_323072963.1 LPS export ABC transporter periplasmic protein LptC -
  IHE33_RS10850 (IHE33_10710) lptA 2463257..2463925 (+) 669 WP_338926979.1 lipopolysaccharide transport periplasmic protein LptA -
  IHE33_RS10855 (IHE33_10715) lptB 2463922..2464701 (+) 780 WP_338925858.1 LPS export ABC transporter ATP-binding protein -
  IHE33_RS10860 (IHE33_10720) rpoN/rpoN1 2464873..2466396 (+) 1524 WP_338925859.1 RNA polymerase factor sigma-54 Machinery gene
  IHE33_RS10865 (IHE33_10725) raiA 2466584..2466934 (+) 351 WP_104076581.1 ribosome-associated translation inhibitor RaiA -
  IHE33_RS10870 (IHE33_10730) - 2467188..2467643 (+) 456 WP_323072966.1 PTS sugar transporter subunit IIA -
  IHE33_RS10875 (IHE33_10735) hprK 2467741..2468709 (+) 969 WP_041754506.1 HPr(Ser) kinase/phosphatase -
  IHE33_RS10880 (IHE33_10740) rapZ 2468763..2469677 (+) 915 WP_323073434.1 RNase adapter RapZ -
  IHE33_RS10885 (IHE33_10745) - 2469703..2470344 (+) 642 WP_323072967.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 507 a.a.        Molecular weight: 55376.72 Da        Isoelectric Point: 5.2634

>NTDB_id=486751 IHE33_RS10860 WP_338925859.1 2464873..2466396(+) (rpoN/rpoN1) [Mycetohabitans endofungorum strain B60]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENEDDWIASPLRVAADGSLLSSQSSALSTGMAE
APAPVLGSASGVNSERAESAEPAAVDEYNGMNSDSGTDTASWNLDDYVRPGGTSDDDDMPPLQLHESTVSLREHLGAQLR
LTKASPRDRALVTFLIESLDDDGYLSASCNEILADLPAELEVDADELNAALALLHSFDPAGVGARSASECLKLQLLRLEP
SATRTLALEIVSNYLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKTSQGWQAEL
NREVVPRLRVNHLYANILRSNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQRSFFVHGEIAMRPLVLRE
IADTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQ
GFVIARRTVAKYREALKIPAVSMRKSL

Nucleotide


Download         Length: 1524 bp        

>NTDB_id=486751 IHE33_RS10860 WP_338925859.1 2464873..2466396(+) (rpoN/rpoN1) [Mycetohabitans endofungorum strain B60]
ATGAAAGCAAGCCTTCAACTCCGTCTATCGCAGCATCTGGCACTGACCCCGCAATTGCAGCAGTCGATTCGGCTACTGCA
GCTGTCGACGCTCGAATTGCAGCAGGAGGTCGCCACGGCGATCGCGCAAAATCCTTTACTGGAGAACGAGGACGACTGGA
TCGCGAGCCCGTTGCGGGTCGCCGCGGACGGCTCACTGTTGTCCTCGCAAAGCAGCGCGCTGTCCACCGGCATGGCCGAG
GCGCCAGCACCGGTGCTCGGCAGCGCCAGCGGCGTGAACAGCGAGCGCGCGGAAAGCGCGGAGCCGGCCGCCGTCGACGA
ATACAACGGCATGAACAGCGACAGCGGCACCGACACGGCGTCGTGGAACTTGGACGACTATGTGCGTCCCGGTGGCACCT
CCGACGACGACGACATGCCGCCGCTGCAGTTGCACGAATCCACGGTCAGCCTACGCGAGCATTTGGGCGCGCAGTTGCGC
CTGACCAAGGCTAGCCCGCGCGACCGCGCGCTGGTCACGTTTCTGATCGAGTCGCTGGACGACGATGGCTACCTGAGCGC
GAGCTGCAACGAAATCCTCGCCGACTTGCCGGCCGAACTTGAGGTCGACGCCGATGAGTTGAACGCGGCGCTCGCGCTGC
TGCACAGCTTCGATCCGGCTGGGGTCGGGGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAATTGTTGCGGCTGGAGCCA
AGCGCCACGCGCACGCTGGCGCTGGAAATCGTGTCGAACTACCTGGAGTTGCTGGCGGCGCGCGACTTCACGCGTCTGCG
CAAGCACCTGAAGGCCAGCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCCG
CGTACGGCAAGGCCGAGGCCGACTACGTGGTGCCGGACATCATCGTGAAAAAAACCTCACAGGGGTGGCAGGCGGAGTTG
AACCGCGAAGTCGTGCCGCGGCTGCGGGTCAACCACTTATACGCGAACATCCTGCGCAGCAACCGGGGTGACCCAGGCAG
CGGTTCGTTGCGCCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAACATCCAGCAACGATTTGAAACGATTTTGCGCG
TCGCACAAGCAATCGTCGAGCGTCAGCGCAGTTTTTTCGTGCACGGCGAGATCGCGATGCGCCCCTTGGTTTTACGAGAA
ATTGCTGATACGCTGGGCTTGCATGAGTCGACCGTATCGCGAGTCACGACCGGGAAGTACATGCTGACGCCCTTTGGGAC
GCTCGAGTTCAAGTACTTCTTTGGCTCGCACGTGTCGACCGATACGGGGGGCGCGGCGTCATCGACCGCGATCCGCGCGC
TCATCAAGCAACTGATAGGAGCCGAAGACCCGAAATCACCTCTTTCAGACAGCCGCATCGCTGAACTGCTGGCCGAACAG
GGCTTCGTGATCGCACGCCGAACTGTGGCGAAATATCGCGAAGCGCTGAAGATTCCGGCTGTTAGCATGCGTAAGTCGTT
ATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(144-334)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(350-505)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.653

100

0.677