Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IG196_RS01345 Genome accession   NZ_CP062121
Coordinates   306215..307807 (+) Length   530 a.a.
NCBI ID   WP_192194671.1    Uniprot ID   A0A7L8SGU7
Organism   Variovorax sp. 38R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 301215..312807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG196_RS01320 (IG196_01320) argS 301292..302977 (+) 1686 WP_192194667.1 arginine--tRNA ligase -
  IG196_RS01325 (IG196_01325) - 302992..303762 (+) 771 WP_192194668.1 SPOR domain-containing protein -
  IG196_RS01330 (IG196_01330) - 303917..304561 (+) 645 WP_192194669.1 thiol:disulfide interchange protein DsbA/DsbL -
  IG196_RS01335 (IG196_01335) lptA 304681..305403 (+) 723 WP_192194670.1 lipopolysaccharide transport periplasmic protein LptA -
  IG196_RS01340 (IG196_01340) lptB 305400..306197 (+) 798 WP_101488358.1 LPS export ABC transporter ATP-binding protein -
  IG196_RS01345 (IG196_01345) rpoN/rpoN1 306215..307807 (+) 1593 WP_192194671.1 RNA polymerase factor sigma-54 Machinery gene
  IG196_RS01350 (IG196_01350) - 307818..309413 (+) 1596 WP_192194672.1 phospholipase D family protein -
  IG196_RS01355 (IG196_01355) - 309418..310062 (-) 645 WP_153280969.1 LysE family translocator -
  IG196_RS01360 (IG196_01360) - 310085..310720 (-) 636 WP_192196885.1 YceI family protein -
  IG196_RS01365 (IG196_01365) - 310765..311346 (-) 582 WP_192194673.1 YceI family protein -
  IG196_RS01370 (IG196_01370) - 311343..311954 (-) 612 WP_225616885.1 cytochrome b -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58460.79 Da        Isoelectric Point: 4.6871

>NTDB_id=486137 IG196_RS01345 WP_192194671.1 306215..307807(+) (rpoN/rpoN1) [Variovorax sp. 38R]
MKQGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDENPFLERTAEEAAREEFGLDAVDTPVPRDDGGDSGEGD
FAPSAPSASTASSEATTSTPESDGPAAEITEREPDWEGDGTVEMAPDDSEWGSDAPARQNNLGDDERADATELARSQESL
QSFLHRQALTLRLNENDCAALRFLIESLNDDGYLEDSLPALASGLAGDDNDQFDDLVHHFQVALGLLQSLEPVGVGARDL
GECLSIQLKALANEDETEAEALVRKTAIAICKQPMELLARRDFKRLATLTRTNEELVRSALQIIARLEPKPGRRFVDVER
NIVVPDVIVTKTGRGTNIKFRVMLNPEVMPRLRVHDIYAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSYFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMSTPFGTVELKYFFGSALGTETGGNASSTAVRALIKQ
FVSSESIKKPLSDSQISEMLKEQGIECARRTVAKYREALRIAPANLRKAL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=486137 IG196_RS01345 WP_192194671.1 306215..307807(+) (rpoN/rpoN1) [Variovorax sp. 38R]
ATGAAGCAAGGGCTGTCCCTTCGCGTCTCGCAGCATCTGGCGCTCACGCCGCAGCTGCAGCAGTCCATTCGCCTGCTCCA
ACTCTCCACGCTCGAACTGAGCCAGGAGGTGGAGCAGATGCTGGACGAGAACCCGTTCCTCGAACGCACCGCGGAAGAAG
CGGCGCGCGAGGAATTCGGGCTCGATGCTGTCGACACGCCCGTGCCGCGCGACGACGGTGGTGACAGCGGCGAGGGCGAT
TTCGCGCCGTCCGCCCCCAGTGCCTCCACCGCTTCCAGCGAAGCGACCACGTCCACACCGGAATCCGACGGCCCCGCCGC
CGAGATCACCGAGCGCGAGCCCGACTGGGAAGGCGACGGCACCGTCGAGATGGCGCCCGACGACAGCGAGTGGGGCAGCG
ACGCGCCCGCACGCCAGAACAACCTCGGCGATGACGAGCGCGCCGACGCCACCGAACTCGCGCGCAGCCAGGAGTCGCTG
CAATCGTTCCTGCACCGCCAGGCGCTGACCCTGCGGCTCAACGAAAACGACTGCGCCGCGCTGCGCTTTTTGATCGAATC
GCTCAACGACGACGGCTACCTCGAAGACTCGCTGCCCGCATTGGCCTCGGGCCTGGCGGGCGACGACAACGACCAGTTCG
ACGACCTCGTGCACCACTTTCAGGTGGCGCTCGGCCTGCTGCAGAGTCTGGAGCCCGTCGGTGTCGGCGCGCGCGACCTC
GGCGAATGCCTGAGCATCCAGCTGAAGGCGCTCGCGAACGAAGACGAGACCGAGGCCGAAGCGCTCGTGCGCAAGACCGC
CATCGCGATCTGCAAGCAGCCGATGGAGCTGCTCGCGCGGCGCGACTTCAAGCGCCTGGCCACGCTCACGCGCACGAACG
AAGAGCTGGTGCGCTCGGCGCTGCAGATCATTGCGCGCCTGGAGCCCAAGCCGGGCCGCCGTTTTGTCGACGTCGAACGC
AACATCGTCGTGCCCGACGTGATCGTCACCAAGACGGGCCGCGGCACCAACATCAAGTTCCGCGTCATGCTGAACCCCGA
GGTCATGCCGCGCCTGCGCGTGCACGACATCTACGCCGGTGCGCTCAAGGCGCACAAGGGCGAGGGCAGCCAGGCGCTGT
CGCAGCGGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGAGCAACGCC
ATCGTCGAACGGCAGAAGAGCTACTTCGTGCACGGCGAACTCGCGATGCGCCCGCTGGTGCTGCGCGAGATCGCCGACGA
GCTGGGCCTGCACGAATCGACCATCTCGCGCGTGACCACCGCCAAGTACATGTCGACGCCCTTCGGCACCGTCGAGCTCA
AGTACTTCTTCGGTTCGGCGCTGGGCACCGAGACCGGCGGCAACGCGTCGAGCACAGCAGTGCGCGCGCTCATCAAGCAG
TTCGTGAGCTCCGAGAGCATCAAGAAGCCGCTGTCCGACAGCCAGATTTCCGAGATGCTGAAAGAGCAGGGCATCGAGTG
CGCGCGCCGCACCGTGGCCAAGTACCGCGAAGCGCTGCGCATCGCGCCCGCCAACCTGCGCAAGGCCCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(372-528)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(151-358)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L8SGU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.469

100

0.508