Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KBW97_RS01910 Genome accession   NZ_CP072986
Coordinates   420743..421420 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain T159A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415743..426420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBW97_RS01885 aph(3')-IIIa 415782..416576 (+) 795 WP_069106888.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
  KBW97_RS01890 tuf 416681..416818 (+) 138 Protein_381 elongation factor Tu -
  KBW97_RS01895 chiA 417110..419803 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  KBW97_RS01900 bfd 419972..420166 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  KBW97_RS01905 bfr 420238..420714 (+) 477 WP_000675504.1 bacterioferritin -
  KBW97_RS01910 pilD 420743..421420 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  KBW97_RS01915 gspM 421420..421881 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  KBW97_RS01920 gspL 421878..423041 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  KBW97_RS01925 gspK 423056..424039 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  KBW97_RS01930 gspJ 424032..424619 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  KBW97_RS01935 gspI 424612..424989 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  KBW97_RS01940 gspH 424986..425495 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  KBW97_RS01945 gspG 425503..425940 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=486078 KBW97_RS01910 WP_000178154.1 420743..421420(-) (pilD) [Escherichia coli strain T159A]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=486078 KBW97_RS01910 WP_000178154.1 420743..421420(-) (pilD) [Escherichia coli strain T159A]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378