Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   KBW66_RS20580 Genome accession   NZ_CP072980
Coordinates   4150705..4152132 (+) Length   475 a.a.
NCBI ID   WP_000980556.1    Uniprot ID   -
Organism   Escherichia coli strain 2547     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4145705..4157132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBW66_RS20540 (KBW66_20540) yeeW 4145889..4146083 (+) 195 WP_000988600.1 DUF5983 family protein -
  KBW66_RS20545 (KBW66_20545) - 4146129..4146209 (+) 81 Protein_4075 hypothetical protein -
  KBW66_RS27125 (KBW66_20550) - 4146498..4146671 (-) 174 WP_032082634.1 transposase domain-containing protein -
  KBW66_RS27130 (KBW66_20555) - 4146698..4146880 (+) 183 WP_001296208.1 EutP/PduV family microcompartment system protein -
  KBW66_RS20560 (KBW66_20560) tmaR 4146981..4147310 (-) 330 WP_000450409.1 DUF496 family protein -
  KBW66_RS20565 (KBW66_20565) yeeA 4147482..4148540 (-) 1059 WP_001200889.1 FUSC family protein -
  KBW66_RS20570 (KBW66_20570) sbmC 4148738..4149211 (-) 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
  KBW66_RS20575 (KBW66_20575) dacD 4149330..4150496 (-) 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  KBW66_RS20580 (KBW66_20580) sbcB 4150705..4152132 (+) 1428 WP_000980556.1 exodeoxyribonuclease I Machinery gene
  KBW66_RS20585 (KBW66_20585) tnpA 4152243..4152701 (-) 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -
  KBW66_RS20590 (KBW66_20590) plaP 4152918..4154276 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  KBW66_RS20595 (KBW66_20595) yoeI 4154266..4154328 (-) 63 WP_010723108.1 membrane protein YoeI -
  KBW66_RS20600 (KBW66_20600) yeeY 4154543..4155472 (-) 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
  KBW66_RS20605 (KBW66_20605) yeeZ 4155518..4156342 (-) 825 WP_000754737.1 SDR family oxidoreductase -
  KBW66_RS20610 (KBW66_20610) yoeB 4156425..4156679 (-) 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  KBW66_RS20615 (KBW66_20615) yefM 4156676..4156927 (-) 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54437.70 Da        Isoelectric Point: 5.4700

>NTDB_id=486052 KBW66_RS20580 WP_000980556.1 4150705..4152132(+) (sbcB) [Escherichia coli strain 2547]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVILVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=486052 KBW66_RS20580 WP_000980556.1 4150705..4152132(+) (sbcB) [Escherichia coli strain 2547]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTAGTTCACGTTTCCGGAATGTTTGGGGCATGGCGT
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTTTGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACACCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTATGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTGA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583