Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IGB12_RS01935 Genome accession   NZ_CP062068
Coordinates   421001..422380 (+) Length   459 a.a.
NCBI ID   WP_004896725.1    Uniprot ID   Q74L28
Organism   Lactobacillus johnsonii strain GHZ10a     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 416001..427380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGB12_RS01915 (IGB12_01915) - 416637..417650 (-) 1014 WP_014567121.1 sugar transferase -
  IGB12_RS01920 (IGB12_01920) pepC 417934..419283 (-) 1350 WP_014567122.1 aminopeptidase C -
  IGB12_RS09995 - 419480..419860 (+) 381 WP_232970587.1 hypothetical protein -
  IGB12_RS10000 - 419879..420331 (+) 453 WP_232970588.1 hypothetical protein -
  IGB12_RS01930 (IGB12_01930) - 420450..421001 (+) 552 WP_004896724.1 dUTP diphosphatase -
  IGB12_RS01935 (IGB12_01935) radA 421001..422380 (+) 1380 WP_004896725.1 DNA repair protein RadA Machinery gene
  IGB12_RS01940 (IGB12_01940) gltX 422462..423961 (+) 1500 WP_004895777.1 glutamate--tRNA ligase -
  IGB12_RS01945 (IGB12_01945) cysS 424051..425463 (+) 1413 WP_004896726.1 cysteine--tRNA ligase -
  IGB12_RS01950 (IGB12_01950) - 425456..425899 (+) 444 WP_004895772.1 ribonuclease III domain-containing protein -
  IGB12_RS01955 (IGB12_01955) rlmB 425886..426641 (+) 756 WP_004895769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50118.70 Da        Isoelectric Point: 7.6366

>NTDB_id=486011 IGB12_RS01935 WP_004896725.1 421001..422380(+) (radA) [Lactobacillus johnsonii strain GHZ10a]
MAKVKTRYKCRNCGYISASYLGRCPNCGAWNQFEEETQEIKKVSTKATASRLMTKIGNNDPVKLTEVKAEKEKRIVTPFE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQITGALAKEHSVLYVSGEESASQIKMRADRLGVSNSGILLYPETNMQNI
RDQIDEIKPDFLVIDSIQTMNEPSLDSMVGSASQVREVTSELMKIAKNEQITTFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDGHHSYRILRSVKNRFGAANEIGMFEMKNEGLAEVSNPSAIFLDERLPNSTGSSVVVSLEGTRPLLADIQALVTPTA
FGYAKRTTSGLDFNRVALLLAVLEKRGNLMLQNQDAFLTATGGIKLNEPAIDLAICMAVASSYKNKEISSTDCFVGEVGL
TGEIRRVNQIEARVKEAAKVGFKRIFIPKNNLNQELKNNSEIEVIGVASLPQALKLVFN

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=486011 IGB12_RS01935 WP_004896725.1 421001..422380(+) (radA) [Lactobacillus johnsonii strain GHZ10a]
ATGGCAAAAGTTAAAACTCGTTATAAGTGTCGTAATTGTGGATATATTTCTGCTTCCTATCTTGGAAGATGTCCCAATTG
TGGTGCTTGGAATCAATTTGAAGAAGAGACACAGGAAATTAAAAAAGTATCAACTAAGGCAACTGCAAGTCGGTTGATGA
CTAAAATTGGAAATAATGATCCAGTAAAGTTAACTGAAGTAAAAGCTGAGAAAGAAAAAAGAATTGTGACTCCTTTTGAA
GAATTAAATCGAGTTTTAGGAGGAGGGATTGTTCCAGGATCTTTAGTTTTAATTGGAGGAGATCCAGGAATTGGAAAATC
AACCTTAATGCTTCAAATTACTGGTGCTTTAGCTAAAGAACATAGCGTCTTATATGTTTCAGGAGAAGAGTCCGCAAGTC
AAATAAAGATGCGGGCAGATCGATTAGGAGTAAGCAATAGCGGAATTTTGCTTTATCCAGAAACAAATATGCAGAATATT
CGTGACCAGATTGATGAAATTAAGCCAGATTTTTTGGTAATTGATTCTATTCAAACAATGAATGAACCTTCACTTGATTC
AATGGTTGGATCTGCTTCTCAAGTACGTGAAGTAACAAGTGAACTAATGAAAATTGCTAAAAATGAACAAATAACTACTT
TTGTTATTGGCCATGTAACTAAAGAAGGAGCAATTGCTGGGCCTAAGATTATGGAACATATGGTTGATACCGTTCTTTAC
TTTGAAGGAGATGGGCACCACTCATATCGAATTTTAAGATCAGTAAAGAATCGTTTTGGTGCAGCTAATGAAATTGGAAT
GTTTGAGATGAAAAATGAAGGGTTAGCAGAAGTAAGTAATCCTTCAGCTATTTTCTTAGACGAACGCTTACCTAATTCGA
CTGGTTCCTCAGTTGTTGTTTCACTTGAAGGAACGCGGCCTCTTCTTGCGGATATCCAAGCTTTAGTCACCCCAACAGCA
TTCGGATATGCAAAAAGAACAACTTCAGGATTAGATTTTAATCGAGTAGCACTTTTATTGGCAGTTTTAGAAAAAAGAGG
CAACTTAATGCTTCAAAATCAAGATGCTTTTCTAACTGCAACTGGTGGGATTAAATTAAATGAACCAGCAATTGATCTTG
CAATTTGCATGGCAGTTGCTTCAAGTTATAAAAATAAAGAAATTTCTTCGACTGATTGCTTTGTAGGTGAAGTTGGCTTG
ACTGGTGAAATTAGAAGAGTTAATCAAATTGAAGCTAGAGTTAAAGAGGCCGCAAAAGTAGGCTTTAAGCGAATTTTTAT
TCCTAAAAACAACTTAAACCAGGAGCTTAAGAATAACTCTGAGATCGAAGTAATTGGAGTAGCAAGTTTACCACAAGCTT
TAAAACTTGTTTTTAACTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q74L28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

60.262

99.782

0.601
  radA Streptococcus pneumoniae Rx1

60.262

99.782

0.601
  radA Streptococcus pneumoniae D39

60.262

99.782

0.601
  radA Streptococcus pneumoniae R6

60.262

99.782

0.601
  radA Streptococcus pneumoniae TIGR4

60.262

99.782

0.601
  radA Streptococcus mitis SK321

60.262

99.782

0.601
  radA Bacillus subtilis subsp. subtilis str. 168

56.14

99.346

0.558