Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   IEN91_RS18765 Genome accession   NZ_CP061938
Coordinates   3774847..3775992 (+) Length   381 a.a.
NCBI ID   WP_003151067.1    Uniprot ID   A0A6A8LBV3
Organism   Bacillus velezensis strain FIO1408     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3769847..3780992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEN91_RS18745 (IEN91_18745) - 3770321..3771037 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  IEN91_RS18750 (IEN91_18750) narK 3771135..3772325 (-) 1191 WP_196616142.1 nitrate transporter NarK -
  IEN91_RS18755 (IEN91_18755) argS 3772460..3774130 (-) 1671 WP_014306019.1 arginine--tRNA ligase -
  IEN91_RS18760 (IEN91_18760) - 3774127..3774555 (-) 429 WP_003151069.1 DUF1934 domain-containing protein -
  IEN91_RS18765 (IEN91_18765) rapF 3774847..3775992 (+) 1146 WP_003151067.1 tetratricopeptide repeat protein Regulator
  IEN91_RS18770 (IEN91_18770) - 3775976..3776095 (+) 120 WP_003151066.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  IEN91_RS18775 (IEN91_18775) - 3776294..3776497 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  IEN91_RS18780 (IEN91_18780) - 3776511..3776714 (+) 204 WP_003151062.1 hypothetical protein -
  IEN91_RS18785 (IEN91_18785) - 3777122..3777403 (+) 282 WP_014306021.1 hypothetical protein -
  IEN91_RS18790 (IEN91_18790) - 3777447..3777959 (+) 513 WP_015387558.1 stage II sporulation protein M -
  IEN91_RS18795 (IEN91_18795) - 3778550..3778768 (-) 219 WP_003151056.1 helix-turn-helix transcriptional regulator -
  IEN91_RS18800 (IEN91_18800) speB 3778938..3779810 (-) 873 WP_003151054.1 agmatinase -
  IEN91_RS18805 (IEN91_18805) speE 3779870..3780700 (-) 831 WP_196616143.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45339.49 Da        Isoelectric Point: 4.9293

>NTDB_id=485625 IEN91_RS18765 WP_003151067.1 3774847..3775992(+) (rapF) [Bacillus velezensis strain FIO1408]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELDQREYLSAIKFFKKAETKLSFIKDTIDKAEYFFKMSEVYYYLKQTYVS
TDYARQAYEIYKAQSSYDIRLIQCQSLFGANFLDLKLYDRAITHFQKAFSLAETEKQPQLMGRTLYNIALCKQSLNDYEA
AITYVEKAIDVFESAKMTPSLPQAYFLMTQIHYKLERYDKALEYHKKGVIYSEESADSVYLHEFEFLKSLYVNGPDEDAM
KRSFDFLESKMMYADIEDFALDAAKYYHKLEDYKKASTYFLKVEEARQHIQGGVTFYEAEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=485625 IEN91_RS18765 WP_003151067.1 3774847..3775992(+) (rapF) [Bacillus velezensis strain FIO1408]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGATCAGCGGGAATATTTGTCGGCTATAAAATTTTTTAAAAAGGCTGAGACGAAACTCTCATTTA
TTAAGGATACTATTGATAAAGCCGAGTACTTTTTCAAGATGTCTGAAGTCTACTACTATTTGAAACAAACGTATGTATCT
ACCGATTACGCAAGGCAGGCATATGAAATATATAAAGCTCAGTCGTCATACGATATCAGGCTCATACAATGCCAATCCTT
ATTCGGAGCAAATTTCCTGGATTTAAAACTATATGATCGGGCAATTACTCACTTTCAAAAGGCTTTTTCTTTGGCAGAAA
CAGAAAAACAGCCTCAATTAATGGGCAGGACGCTGTATAACATCGCACTTTGCAAACAAAGCCTGAACGACTATGAAGCA
GCTATAACTTATGTTGAAAAAGCCATTGATGTTTTTGAAAGTGCTAAAATGACCCCTTCTTTGCCCCAAGCATATTTTTT
AATGACACAGATTCATTATAAATTAGAAAGATACGACAAAGCGCTCGAGTATCATAAAAAAGGAGTTATCTATTCCGAAG
AGTCAGCAGATTCAGTTTATTTACACGAATTCGAATTTTTAAAATCTTTGTATGTGAACGGACCTGATGAGGATGCAATG
AAACGGAGTTTTGACTTTTTGGAAAGTAAGATGATGTATGCAGATATAGAAGATTTTGCTCTTGATGCAGCTAAATATTA
TCACAAACTTGAAGATTATAAAAAAGCTTCAACATACTTTTTAAAAGTTGAAGAAGCAAGACAGCATATTCAAGGGGGGG
TAACTTTTTATGAAGCTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(223-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LBV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

74.803

100

0.748
  rapC Bacillus subtilis subsp. subtilis str. 168

53.158

99.738

0.53