Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IEN91_RS01775 Genome accession   NZ_CP061938
Coordinates   359328..359765 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain FIO1408     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 354328..364765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEN91_RS20735 - 354910..355038 (-) 129 Protein_303 hypothetical protein -
  IEN91_RS20990 (IEN91_01740) - 355186..356387 (+) 1202 Protein_304 GTP-binding protein -
  IEN91_RS01745 (IEN91_01745) - 356452..357588 (+) 1137 WP_196616220.1 zinc-dependent alcohol dehydrogenase -
  IEN91_RS01750 (IEN91_01750) - 357603..358037 (+) 435 WP_003156592.1 RDD family protein -
  IEN91_RS01755 (IEN91_01755) - 358154..358429 (+) 276 WP_369719109.1 YckD family protein -
  IEN91_RS01760 (IEN91_01760) - 358533..358649 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  IEN91_RS01765 (IEN91_01765) - 358648..358867 (+) 220 Protein_309 family 1 glycosylhydrolase -
  IEN91_RS01770 (IEN91_01770) nin/comJ 358908..359306 (-) 399 WP_043866822.1 competence protein ComJ Regulator
  IEN91_RS01775 (IEN91_01775) nucA/comI 359328..359765 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  IEN91_RS01780 (IEN91_01780) hxlB 360124..360681 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  IEN91_RS01785 (IEN91_01785) hxlA 360678..361313 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  IEN91_RS01790 (IEN91_01790) - 361545..361907 (+) 363 WP_014304320.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=485562 IEN91_RS01775 WP_003156588.1 359328..359765(-) (nucA/comI) [Bacillus velezensis strain FIO1408]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=485562 IEN91_RS01775 WP_003156588.1 359328..359765(-) (nucA/comI) [Bacillus velezensis strain FIO1408]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641