Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   J9312_RS13120 Genome accession   NZ_CP072845
Coordinates   2436827..2437237 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain XP     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2431827..2442237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9312_RS13090 (J9312_13010) - 2431914..2432054 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  J9312_RS13095 (J9312_13015) yqeF 2432360..2433091 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  J9312_RS13100 (J9312_13020) cwlH 2433343..2434095 (-) 753 WP_264379321.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  J9312_RS13105 (J9312_13025) yqeD 2434282..2434908 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  J9312_RS13110 (J9312_13030) gnd 2434927..2435820 (-) 894 WP_069837643.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  J9312_RS13115 (J9312_13035) yqeB 2436072..2436794 (+) 723 WP_014480321.1 hypothetical protein -
  J9312_RS13120 (J9312_13040) nucA/comI 2436827..2437237 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  J9312_RS13125 (J9312_13045) sigK 2437433..2438161 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  J9312_RS13130 (J9312_13050) - 2438161..2438259 (+) 99 WP_031600702.1 hypothetical protein -
  J9312_RS13135 - 2438256..2438468 (-) 213 Protein_2537 recombinase family protein -
  J9312_RS13140 (J9312_13060) fumC 2438687..2440075 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  J9312_RS13145 (J9312_13065) - 2440242..2441132 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=485491 J9312_RS13120 WP_009967785.1 2436827..2437237(-) (nucA/comI) [Bacillus subtilis strain XP]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=485491 J9312_RS13120 WP_009967785.1 2436827..2437237(-) (nucA/comI) [Bacillus subtilis strain XP]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529