Detailed information NTG0485475

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: IE382_RS22360
• Genome accession: NZ_CP061870
• Coordinates: 4160044..4161189 (+)
• Length: 381 a.a.
• NCBI ID: WP_017696253.1
• Uniprot ID: A0A0C3JF17
• Organism: Bacillus subtilis strain FX-1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 4155044..4166189

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IE382_RS22340 (IE382_22240)	albF	4155060..4156340 (+)	1281	WP_017696258.1	pitrilysin family protein	-
IE382_RS22345 (IE382_22245)	albG	4156337..4157038 (+)	702	WP_029318695.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
IE382_RS22350 (IE382_22250)	ywhL	4157044..4158420 (-)	1377	WP_041351296.1	YncE family protein	-
IE382_RS22355 (IE382_22255)	ywhK	4158459..4159814 (-)	1356	WP_242304441.1	YncE family protein	-
IE382_RS23755	-	4159811..4160041 (-)	231	WP_043858872.1	hypothetical protein	-
IE382_RS22360 (IE382_22260)	rapF	4160044..4161189 (+)	1146	WP_017696253.1	tetratricopeptide repeat protein	Regulator
IE382_RS22365 (IE382_22265)	-	4161173..4161292 (+)	120	WP_014665774.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
IE382_RS22370 (IE382_22270)	ywhH	4161391..4161856 (+)	466	Protein_4360	YbaK/EbsC family protein	-
IE382_RS22375 (IE382_22275)	speB	4161888..4162760 (-)	873	WP_095844026.1	agmatinase	-
IE382_RS22380 (IE382_22280)	speE	4162821..4163651 (-)	831	WP_003227543.1	spermidine synthase	-
IE382_RS22385 (IE382_22285)	pbpG	4163853..4165928 (+)	2076	WP_015384942.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45593.24 Da
• Isoelectric Point: 5.0684

>NTDB_id=485475 IE382_RS22360 WP_017696253.1 4160044..4161189(+) (rapF) [Bacillus subtilis strain FX-1]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDRREYVLAIQFFKKAESKLVFVKDKIDKAEFFFKMSEVYYYMKQTYVS
MDYARQAFEIYSEQTTYDIRMIQCQSLFGANFLDLKQYEKAIAHFQKAFTLAKTEQQPQLMGRTLYNIALCKQNQNSFES
AIEYVKKSIQVFEGSKMLPSLPQAFFLITQIYFKLGKIDNACEYHKKGKEYSAAAGDTVYLTEFEFLESLYLSGPDEERI
EKCFVFLESKMMYADIEDFALDVAKYYHQQENYKKASHYFLKVEEARQHIQGGVNLYEMEV

Nucleotide

• Length: 1146 bp

>NTDB_id=485475 IE382_RS22360 WP_017696253.1 4160044..4161189(+) (rapF) [Bacillus subtilis strain FX-1]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGACCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGATCGCCGTGAATATGTATTAGCCATTCAATTCTTTAAAAAAGCTGAGAGCAAGCTGGTTTTTG
TCAAAGATAAAATCGATAAAGCTGAATTCTTTTTCAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTCTCC
ATGGACTATGCACGTCAAGCATTCGAAATATACAGTGAACAGACTACATATGATATCAGAATGATTCAATGCCAGTCCTT
ATTCGGAGCCAACTTTTTGGATTTAAAACAATACGAAAAAGCAATTGCCCATTTTCAAAAAGCCTTTACACTTGCAAAAA
CAGAGCAGCAGCCCCAATTAATGGGAAGAACACTTTATAACATAGCACTCTGCAAGCAAAACCAAAACAGTTTTGAATCA
GCGATAGAGTATGTAAAAAAATCTATTCAAGTATTTGAAGGATCTAAAATGCTTCCTTCATTGCCACAAGCTTTTTTCCT
AATTACACAAATTTATTTTAAATTAGGTAAGATTGATAACGCCTGTGAATATCATAAAAAAGGAAAAGAGTATTCTGCAG
CTGCGGGAGATACCGTGTATCTAACGGAGTTTGAATTTTTGGAATCTTTATACTTGTCTGGACCGGATGAGGAACGGATA
GAAAAATGTTTTGTTTTTCTTGAAAGTAAAATGATGTATGCAGATATTGAGGACTTCGCACTTGATGTTGCTAAATATTA
TCATCAACAGGAAAATTACAAAAAAGCATCTCATTACTTTTTAAAGGTTGAAGAAGCAAGACAGCACATTCAAGGGGGGG
TGAATTTATATGAAATGGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_8) (263-290)

• Tetratricopeptide repeat (TPR_12) (189-253)

3D structure

• AlphaFold DB: A0A0C3JF17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	80.052	100	0.801
rapC	Bacillus subtilis subsp. subtilis str. 168	57.105	99.738	0.57