Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IE382_RS03405 Genome accession   NZ_CP061870
Coordinates   640520..640969 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain FX-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 635520..645969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE382_RS03370 (IE382_03355) yckA 635795..636475 (-) 681 WP_242304515.1 amino acid ABC transporter permease -
  IE382_RS03375 (IE382_03360) yckB 636485..637348 (-) 864 WP_237983781.1 transporter substrate-binding domain-containing protein -
  IE382_RS03380 (IE382_03365) - 637511..637701 (+) 191 Protein_624 hypothetical protein -
  IE382_RS03385 (IE382_03370) - 637746..638057 (+) 312 Protein_625 RDD family protein -
  IE382_RS03390 (IE382_03375) yckD 638139..638471 (+) 333 WP_015252849.1 YckD family protein -
  IE382_RS03395 (IE382_03380) bglC 638625..640058 (+) 1434 WP_017696125.1 6-phospho-beta-glucosidase -
  IE382_RS03400 (IE382_03385) nin/comJ 640095..640493 (-) 399 WP_017696126.1 DNA-entry nuclease inhibitor Regulator
  IE382_RS03405 (IE382_03390) nucA/comI 640520..640969 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  IE382_RS03410 (IE382_03395) tlpC 641137..642858 (-) 1722 Protein_630 methyl-accepting chemotaxis protein TlpC -
  IE382_RS03415 (IE382_03400) hxlB 642969..643526 (-) 558 WP_017696129.1 6-phospho-3-hexuloisomerase -
  IE382_RS03420 (IE382_03405) hxlA 643532..644164 (-) 633 WP_017696130.1 3-hexulose-6-phosphate synthase -
  IE382_RS03425 (IE382_03410) hxlR 644396..644758 (+) 363 WP_017696131.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=485410 IE382_RS03405 WP_014475770.1 640520..640969(-) (nucA/comI) [Bacillus subtilis strain FX-1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=485410 IE382_RS03405 WP_014475770.1 640520..640969(-) (nucA/comI) [Bacillus subtilis strain FX-1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987