Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IE339_RS18825 Genome accession   NZ_CP061868
Coordinates   3577857..3578918 (-) Length   353 a.a.
NCBI ID   WP_242170091.1    Uniprot ID   -
Organism   Priestia koreensis strain FS-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3572857..3583918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE339_RS18780 (IE339_18760) moaA 3573146..3574165 (-) 1020 WP_242170075.1 GTP 3',8-cyclase MoaA -
  IE339_RS18785 (IE339_18765) - 3574243..3574440 (-) 198 WP_053402146.1 YqzE family protein -
  IE339_RS18790 (IE339_18770) - 3574478..3574972 (-) 495 WP_242170078.1 shikimate kinase -
  IE339_RS18795 (IE339_18775) comGG 3574974..3575351 (-) 378 WP_242170081.1 competence type IV pilus minor pilin ComGG -
  IE339_RS18800 (IE339_18780) comGF 3575356..3575781 (-) 426 WP_242170083.1 competence type IV pilus minor pilin ComGF -
  IE339_RS18805 (IE339_18785) - 3575778..3576080 (-) 303 WP_242170085.1 hypothetical protein -
  IE339_RS18810 (IE339_18790) comGD 3576094..3576561 (-) 468 WP_242170087.1 competence type IV pilus minor pilin ComGD -
  IE339_RS18815 (IE339_18795) comGC 3576518..3576817 (-) 300 WP_277933919.1 competence type IV pilus major pilin ComGC -
  IE339_RS18820 (IE339_18800) comGB 3576827..3577864 (-) 1038 WP_242170089.1 competence type IV pilus assembly protein ComGB -
  IE339_RS18825 (IE339_18805) comGA 3577857..3578918 (-) 1062 WP_242170091.1 competence type IV pilus ATPase ComGA Machinery gene
  IE339_RS18830 (IE339_18810) - 3579057..3579443 (-) 387 WP_242170093.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  IE339_RS18835 (IE339_18815) - 3579636..3580331 (+) 696 WP_242170096.1 helix-turn-helix domain-containing protein -
  IE339_RS18840 (IE339_18820) - 3580440..3580682 (+) 243 WP_053402135.1 DUF2626 domain-containing protein -
  IE339_RS18845 (IE339_18825) - 3580715..3581821 (-) 1107 WP_242170098.1 SAM-dependent methyltransferase -
  IE339_RS18850 (IE339_18830) - 3581847..3582479 (-) 633 WP_242170102.1 MBL fold metallo-hydrolase -
  IE339_RS18855 (IE339_18835) - 3582702..3582875 (+) 174 WP_242170104.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 40032.30 Da        Isoelectric Point: 8.5607

>NTDB_id=485380 IE339_RS18825 WP_242170091.1 3577857..3578918(-) (comGA) [Priestia koreensis strain FS-1]
MQEIEQLAEYLITDAYRHRVSDIHILPREQDAIVQFRVGYDLVFKESMSKRICERLITHFKFLGGMDIGEKRRPQNGALK
ISIQEDHINLRLSTLPTAYEESLVIRVLPSNHLTPLQKLSLFPNTVTKLIALLKYSHGLLIFTGPTGSGKTTTLYTLLEE
SKNMFNRNIITLEDPIERRSEGVMQVQVNEKAGITYTTGLKAILRHDPDIIMVGEIRDAETAQIAVRAALTGHLVLTTMH
TRDSKGAIYRLLEFGVSYQEIEQTLVGVVAQRLLEIYCPLCGEPCHPVCKRSRKNRQASIYEILTGKNLMSVLHEVNGKE
SSYNYKTLKDAIVKGIALGYVHDSNLSRWVRND

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=485380 IE339_RS18825 WP_242170091.1 3577857..3578918(-) (comGA) [Priestia koreensis strain FS-1]
TTGCAAGAAATTGAACAGCTAGCTGAATATTTGATTACAGACGCTTATCGACACAGAGTGTCAGACATTCATATTTTACC
CAGAGAACAAGACGCTATTGTACAATTTAGGGTGGGATATGATTTAGTATTTAAGGAGTCTATGAGCAAGCGAATATGTG
AACGTCTCATTACTCATTTTAAATTTTTAGGGGGGATGGACATAGGAGAAAAAAGAAGACCGCAAAATGGCGCTTTAAAA
ATTTCAATTCAAGAGGACCATATTAACCTTCGTTTGTCCACGCTTCCAACAGCTTATGAGGAAAGTCTTGTTATTCGAGT
ACTTCCTTCTAACCATCTCACACCACTTCAAAAGCTTTCTTTATTTCCAAACACAGTTACAAAACTCATCGCACTCCTCA
AATATTCACACGGTTTATTAATCTTCACAGGGCCAACCGGCTCCGGAAAAACAACCACTCTTTACACGTTACTTGAAGAA
TCCAAAAATATGTTTAACCGAAATATTATTACACTTGAAGACCCTATTGAACGTAGAAGTGAAGGAGTTATGCAGGTTCA
GGTTAATGAAAAAGCAGGTATCACCTATACGACAGGTTTAAAGGCAATATTGCGCCATGATCCAGACATTATAATGGTAG
GTGAAATACGTGATGCTGAAACAGCACAAATTGCTGTACGCGCTGCGTTAACTGGTCACTTGGTTTTGACTACTATGCAT
ACAAGAGATTCGAAAGGTGCTATCTATAGATTGCTTGAGTTTGGTGTTTCCTATCAAGAAATTGAACAAACGTTAGTGGG
AGTGGTCGCTCAACGTCTGTTAGAAATCTATTGTCCACTTTGTGGGGAGCCTTGTCATCCTGTGTGCAAGCGTAGCCGAA
AAAATAGACAGGCAAGCATTTATGAAATCTTAACAGGGAAAAATTTAATGTCGGTTCTTCATGAAGTAAATGGAAAAGAG
TCCTCGTATAACTATAAAACATTGAAGGATGCGATAGTAAAAGGTATTGCTCTAGGATATGTACATGATTCAAATTTGAG
TAGATGGGTGCGTAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

60.399

99.433

0.601
  pilB Glaesserella parasuis strain SC1401

37.5

99.717

0.374