Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J8617_RS16645 Genome accession   NZ_CP072802
Coordinates   3459560..3460237 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain PK5074     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3454560..3465237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8617_RS16630 (J8617_16625) chiA 3455927..3458620 (+) 2694 WP_000773155.1 bifunctional chitinase/lysozyme -
  J8617_RS16635 (J8617_16630) bfd 3458789..3458983 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J8617_RS16640 (J8617_16635) bfr 3459055..3459531 (+) 477 WP_000675504.1 bacterioferritin -
  J8617_RS16645 (J8617_16640) pilD 3459560..3460237 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  J8617_RS16650 (J8617_16645) gspM 3460237..3460698 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  J8617_RS16655 (J8617_16650) gspL 3460695..3461858 (-) 1164 WP_001115234.1 type II secretion system protein GspL -
  J8617_RS16660 (J8617_16655) gspK 3461873..3462856 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  J8617_RS16665 (J8617_16660) gspJ 3462849..3463436 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  J8617_RS16670 (J8617_16665) gspI 3463429..3463806 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  J8617_RS16675 (J8617_16670) gspH 3463803..3464312 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  J8617_RS16680 (J8617_16675) gspG 3464320..3464757 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=485368 J8617_RS16645 WP_000178161.1 3459560..3460237(-) (pilD) [Escherichia coli strain PK5074]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=485368 J8617_RS16645 WP_000178161.1 3459560..3460237(-) (pilD) [Escherichia coli strain PK5074]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378