Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   J8617_RS14450 Genome accession   NZ_CP072802
Coordinates   2976709..2978229 (+) Length   506 a.a.
NCBI ID   WP_112023757.1    Uniprot ID   -
Organism   Escherichia coli strain PK5074     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 2971709..2983229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8617_RS14425 (J8617_14420) ilvE 2973263..2974192 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  J8617_RS14430 (J8617_14425) ilvM 2974212..2974475 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  J8617_RS14435 (J8617_14430) ilvG 2974472..2976118 (-) 1647 WP_001012608.1 acetolactate synthase 2 catalytic subunit -
  J8617_RS14440 (J8617_14435) ilvX 2976121..2976171 (-) 51 WP_001387183.1 peptide IlvX -
  J8617_RS14445 (J8617_14440) ilvL 2976258..2976356 (-) 99 WP_001311244.1 ilv operon leader peptide -
  J8617_RS14450 (J8617_14445) comM 2976709..2978229 (+) 1521 WP_112023757.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  J8617_RS14455 (J8617_14450) maoP 2978254..2978592 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  J8617_RS14460 (J8617_14455) hdfR 2978711..2979550 (+) 840 WP_000379248.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55145.41 Da        Isoelectric Point: 8.1909

>NTDB_id=485356 J8617_RS14450 WP_112023757.1 2976709..2978229(+) (comM) [Escherichia coli strain PK5074]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQKQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=485356 J8617_RS14450 WP_112023757.1 2976709..2978229(+) (comM) [Escherichia coli strain PK5074]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCGATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTGGCTCCAGCTGATCTGCCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTATCGGTCAGAAACAAGGAAAGCGAGGACTGGAAATTACCGCTGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGTGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCATCGTTAACTGCGATGGTAGGCGGTGGCGCAATTCCAGGGCCCGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAATGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGTTGGAAGGAACACTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATA
A

Domains

Predicted by InterProScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.904

100

0.595
  comM Vibrio cholerae O1 biovar El Tor strain E7946

59.562

99.209

0.591
  comM Vibrio cholerae strain A1552

59.562

99.209

0.591
  comM Glaesserella parasuis strain SC1401

58.777

100

0.589
  comM Vibrio campbellii strain DS40M4

58.367

99.209

0.579
  comM Acinetobacter baylyi ADP1

48.996

98.419

0.482
  comM Legionella pneumophila str. Paris

47.992

98.419

0.472
  comM Legionella pneumophila strain ERS1305867

47.992

98.419

0.472
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.071

100

0.441