Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IEZ30_RS04375 Genome accession   NZ_CP061849
Coordinates   992074..993498 (-) Length   474 a.a.
NCBI ID   WP_107731613.1    Uniprot ID   -
Organism   Chromobacterium haemolyticum strain Bb2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 987074..998498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEZ30_RS04345 (IEZ30_04345) - 987131..988621 (-) 1491 WP_019104264.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
  IEZ30_RS04350 (IEZ30_04350) - 988667..989302 (-) 636 WP_019104263.1 flavin prenyltransferase UbiX -
  IEZ30_RS04355 (IEZ30_04355) rapZ 989337..990179 (-) 843 WP_019104262.1 RNase adapter RapZ -
  IEZ30_RS04360 (IEZ30_04360) hprK 990160..991116 (-) 957 WP_019104261.1 HPr(Ser) kinase/phosphatase -
  IEZ30_RS04365 (IEZ30_04365) ptsN 991109..991570 (-) 462 WP_019104260.1 PTS IIA-like nitrogen regulatory protein PtsN -
  IEZ30_RS04370 (IEZ30_04370) raiA 991692..992006 (-) 315 WP_019104259.1 ribosome-associated translation inhibitor RaiA -
  IEZ30_RS04375 (IEZ30_04375) rpoN/rpoN1 992074..993498 (-) 1425 WP_107731613.1 RNA polymerase factor sigma-54 Machinery gene
  IEZ30_RS04380 (IEZ30_04380) lptB 993502..994230 (-) 729 WP_019104257.1 LPS export ABC transporter ATP-binding protein -
  IEZ30_RS04385 (IEZ30_04385) lptA 994227..994781 (-) 555 WP_043591966.1 lipopolysaccharide transport periplasmic protein LptA -
  IEZ30_RS04390 (IEZ30_04390) lptC 994771..995340 (-) 570 WP_019104255.1 LPS export ABC transporter periplasmic protein LptC -
  IEZ30_RS04395 (IEZ30_04395) - 995337..995861 (-) 525 WP_019104254.1 HAD-IIIA family hydrolase -
  IEZ30_RS04400 (IEZ30_04400) - 995887..996867 (-) 981 WP_039756662.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 52235.23 Da        Isoelectric Point: 4.6805

>NTDB_id=485119 IEZ30_RS04375 WP_107731613.1 992074..993498(-) (rpoN/rpoN1) [Chromobacterium haemolyticum strain Bb2]
MKQTLQLKVSQQLTLTPQLQQSIKLLQMSTLDLQTEVERFLLDNPLLERGDEAPQGEAADAAAGQEQDGAAETRDEASGD
EGERGAEAGELSDWGSGGRRGDGDDELDPMLNVPCPISLREHLLAQLGEVALPTRDQAIVQLLIEELDDDGYLGQTLEDI
ASHLPLELEIEADELMIGLRLLQQFDPPGVGARSLPEALALQLQRLDPDEPQRELALAIVRDHLTLLGNRDYTRLKKQLS
VDDAALRAAQALIARLNPRPTAGFGASDTQYVIPDVSVRKRKGRWVAALNGGAVPRLRVNQMYARLLAENRGGNGEMSSR
LQEAKWLVKNIQQRFDTILKVAEAIVERQQSFFEHGEVAMRPLILRDIADELGLHESTVSRVTTQKYLLCPRGLFELKYF
FGSALETDSGGECSATAIKAHIRSLIDAESPSKPLSDSALVEALSKQGIQVARRTIAKYREAMQIPPVNQRKTL

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=485119 IEZ30_RS04375 WP_107731613.1 992074..993498(-) (rpoN/rpoN1) [Chromobacterium haemolyticum strain Bb2]
ATGAAACAGACTCTTCAGCTCAAGGTATCGCAGCAGCTGACCCTGACGCCGCAGCTGCAGCAGTCGATCAAGCTGCTGCA
GATGTCCACGCTCGACCTGCAAACCGAAGTCGAGCGCTTCTTGCTGGACAATCCCTTGCTTGAGCGCGGCGACGAAGCGC
CGCAGGGCGAAGCGGCTGACGCCGCCGCCGGCCAGGAACAGGATGGCGCCGCGGAAACCCGCGACGAGGCCTCCGGCGAC
GAAGGCGAGCGCGGCGCCGAGGCCGGCGAACTGAGCGACTGGGGCAGCGGCGGCCGCCGCGGCGACGGCGACGACGAACT
GGATCCGATGCTGAACGTGCCCTGTCCAATCAGCCTGCGCGAGCACCTGCTGGCGCAACTGGGCGAAGTGGCGCTGCCTA
CGCGCGACCAGGCCATCGTTCAGTTGCTGATCGAAGAGCTGGACGACGACGGCTACCTGGGCCAAACGCTGGAAGACATC
GCCAGTCACCTGCCGCTGGAACTGGAAATCGAGGCCGACGAACTGATGATAGGCCTGCGCCTGCTGCAACAGTTCGACCC
GCCCGGCGTCGGCGCGCGCTCGCTGCCGGAAGCGCTGGCGCTGCAATTGCAGCGGCTGGACCCGGACGAGCCCCAGCGAG
AACTGGCGCTGGCCATCGTCCGCGACCACCTGACCTTGCTGGGCAACCGAGACTACACCCGGCTGAAAAAGCAGCTGAGC
GTGGACGACGCCGCGCTGCGCGCCGCCCAGGCGCTGATCGCCCGCCTCAACCCCCGCCCCACCGCCGGTTTCGGCGCCAG
CGACACTCAATACGTGATCCCCGACGTCTCGGTCAGAAAACGCAAGGGCCGCTGGGTGGCCGCCCTGAACGGCGGCGCCG
TGCCCAGACTCCGCGTCAACCAGATGTACGCTCGCTTGCTGGCGGAAAATCGCGGCGGCAACGGTGAAATGTCCTCGCGT
CTGCAAGAGGCGAAATGGCTGGTGAAGAACATCCAGCAGCGATTTGACACTATCCTGAAAGTGGCGGAAGCTATCGTTGA
ACGGCAGCAATCGTTCTTTGAACACGGCGAAGTGGCCATGCGCCCGCTGATCCTGCGCGACATCGCGGACGAGTTGGGCC
TGCATGAATCCACGGTTTCCAGGGTGACCACCCAGAAATACCTGCTCTGCCCGCGCGGCCTGTTCGAGCTCAAGTATTTC
TTCGGCAGCGCGCTGGAAACCGACAGCGGCGGCGAGTGCTCCGCCACCGCGATCAAGGCGCATATCCGCAGCCTGATAGA
CGCCGAATCGCCGAGCAAGCCGCTGTCCGACAGCGCCCTGGTTGAAGCGCTGTCCAAACAGGGAATACAAGTTGCCAGAC
GCACGATAGCCAAGTATCGTGAAGCCATGCAGATACCGCCGGTCAACCAGCGCAAGACGCTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-472)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.094

100

0.561