Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IE322_RS00080 Genome accession   NZ_CP061848
Coordinates   15735..17105 (+) Length   456 a.a.
NCBI ID   WP_015271795.1    Uniprot ID   A0A2N5BBJ6
Organism   Pseudomonas asiatica strain C3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 10735..22105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IE322_RS00055 (IE322_00055) - 11611..11766 (+) 156 WP_013974218.1 DUF2474 domain-containing protein -
  IE322_RS00060 (IE322_00060) - 12086..13210 (-) 1125 WP_100412787.1 methyltransferase -
  IE322_RS00065 (IE322_00065) - 13307..14014 (-) 708 WP_015271798.1 autoinducer binding domain-containing protein -
  IE322_RS00070 (IE322_00070) - 14227..15003 (+) 777 WP_015271797.1 ferredoxin--NADP reductase -
  IE322_RS00075 (IE322_00075) mscL 15138..15557 (-) 420 WP_015271796.1 large-conductance mechanosensitive channel protein MscL -
  IE322_RS00080 (IE322_00080) radA 15735..17105 (+) 1371 WP_015271795.1 DNA repair protein RadA Machinery gene
  IE322_RS00085 (IE322_00085) - 17282..18799 (-) 1518 WP_013974213.1 nucleobase:cation symporter-2 family protein -
  IE322_RS00090 (IE322_00090) - 19063..19425 (-) 363 WP_013974212.1 PilZ domain-containing protein -
  IE322_RS00095 (IE322_00095) - 19604..21670 (+) 2067 WP_015271794.1 carbon starvation CstA family protein -
  IE322_RS00100 (IE322_00100) - 21684..21881 (+) 198 WP_013974210.1 YbdD/YjiX family protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48755.20 Da        Isoelectric Point: 6.8985

>NTDB_id=485089 IE322_RS00080 WP_015271795.1 15735..17105(+) (radA) [Pseudomonas asiatica strain C3]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSTGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAVGMPALYVTGEESQQQVAMRSRRLGLPQDQLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=485089 IE322_RS00080 WP_015271795.1 15735..17105(+) (radA) [Pseudomonas asiatica strain C3]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAGTGCGGTGCGACCTTCCCCAAATGGGCCGGCCAGTGTGGCGAATG
CGGGGCCTGGAACACCCTGGTCGAGACCATGATCGAAAGCGGCGGTGCCGCTGCGCCCAGTACCGGCCGTGCCGGCTGGA
CCGGGCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTCGAGGAGATCCCGCGCTTCACCACCAGCAGCACCGAA
CTGGACCGCGTGCTGGGCGGTGGCCTGGTGGATGGCTCGGTGGTGTTGATCGGTGGTGACCCCGGCATCGGCAAATCGAC
CATCCTGCTGCAGACCTTGTGCAACATTGCCGTGGGCATGCCGGCGCTGTATGTGACCGGCGAGGAGTCGCAGCAGCAGG
TGGCCATGCGCTCCCGGCGCCTGGGCCTGCCCCAGGACCAGCTCAAGGTGATGACCGAAACCTGCATCGAGACCATCATC
GCCACGGCCCGCGTCGAGAAACCGCGGGTCATGGTGATCGACTCGATCCAGACCATTTTCACCGAGCAGCTGCAATCGGC
ACCCGGCGGCGTGGCCCAGGTGCGCGAAAGCACGGCGTTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATCTTCC
TGGTCGGCCATGTGACCAAGGAAGGTTCGCTGGCCGGCCCGCGGGTACTCGAGCACATGGTGGACACCGTGCTGTATTTC
GAGGGCGAGTCCGATGGCCGCCTGCGCCTGCTGCGGGCAGTCAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGTGTGTT
CGGCATGACCGACCGGGGCCTGAAGGAGGTGTCCAACCCCTCGGCGATCTTCCTCAACCGCACCCAGGAGGAAGTACCGG
GCAGCGTGGTGATGGCGACCTGGGAGGGCACCCGGCCGATGCTGGTGGAGGTGCAGGCGCTGGTCGACGACAGCCACCTG
GCCAACCCGCGCCGGGTGACCTTGGGCCTTGACCAGAATCGCCTGGCAATGTTGCTGGCGGTGCTGCACCGCCACGGCGG
TATTCCCACCCATGACCAGGACGTGTTCCTCAACGTGGTGGGTGGGGTGAAGGTGCTGGAAACCGCTTCTGACCTGGCGT
TGCTGGCGGCGGTGATGTCCAGCCTGCGCAACCGGCCCCTGGCCCATGGCCTGCTGGTGTTTGGCGAGATCGGCCTGTCT
GGCGAGGTACGGCCGGTGCCCAGTGGTCAGGAGCGTTTGAAGGAGGCCGCCAAGCATGGTTTCAAGCGGGCCATCGTGCC
CAAGGGCAATGCCCCGAAAGAGGCTCCGGCCGGGTTGCAGGTGATTGCCGTTACCCGGCTGGAGCAGGCTCTGGATGCGT
TGTTCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N5BBJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.684

100

0.487
  radA Streptococcus pneumoniae Rx1

46.087

100

0.465
  radA Streptococcus pneumoniae D39

46.087

100

0.465
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.465
  radA Streptococcus pneumoniae R6

46.087

100

0.465
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus mitis SK321

46.053

100

0.461