Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   J8N08_RS03465 Genome accession   NZ_CP072745
Coordinates   704638..706038 (-) Length   466 a.a.
NCBI ID   WP_003512347.1    Uniprot ID   A0A1B9V3Y2
Organism   Agrobacterium tumefaciens strain BIM B-441     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 699638..711038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8N08_RS03445 (J8N08_03435) cysS 699821..701206 (-) 1386 WP_013635827.1 cysteine--tRNA ligase -
  J8N08_RS03450 (J8N08_03440) - 701432..702172 (-) 741 WP_112391595.1 SDR family NAD(P)-dependent oxidoreductase -
  J8N08_RS03455 (J8N08_03445) purF 702323..703816 (-) 1494 WP_003512343.1 amidophosphoribosyltransferase -
  J8N08_RS03460 (J8N08_03450) - 703955..704542 (-) 588 WP_003512345.1 CvpA family protein -
  J8N08_RS03465 (J8N08_03455) radA/sms 704638..706038 (-) 1401 WP_003512347.1 DNA repair protein RadA Machinery gene
  J8N08_RS03470 (J8N08_03460) - 706236..706505 (-) 270 WP_111789329.1 type II toxin-antitoxin system ParD family antitoxin -
  J8N08_RS03475 (J8N08_03465) alr 706571..707746 (-) 1176 WP_333704149.1 alanine racemase -
  J8N08_RS03480 (J8N08_03470) - 707833..708690 (+) 858 WP_174061494.1 AraC family transcriptional regulator -
  J8N08_RS03485 (J8N08_03475) - 708783..709487 (+) 705 WP_003512355.1 AzlC family ABC transporter permease -
  J8N08_RS03490 (J8N08_03480) - 709484..709786 (+) 303 WP_111795920.1 AzlD family protein -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 49183.10 Da        Isoelectric Point: 6.5901

>NTDB_id=484732 J8N08_RS03465 WP_003512347.1 704638..706038(-) (radA/sms) [Agrobacterium tumefaciens strain BIM B-441]
MAKAKTQFVCQNCGTVHTRWAGKCEGCGEWNTIVEEDPMGGIGSGPGKTPKKGRAVALTALSGEIEEAPRIPTGISELDR
ATGGGFVRGSAVLIGGDPGIGKSTLLMQAAAALSRRGHRVIYVSGEEAVAQVRLRAQRLGAAETDVLLAAETNVEDILAT
ISEGKRPDLVIIDSIQTLWSDTADSAPGTVTQVRTGVQAMIRYAKQTGATMVLVGHVTKEGQIAGPRVVEHMVDAVLYFE
GDRGHHYRILRTVKNRFGPTDEIGVFEMSDRGLREVSNPSELFLGERNEKSPGAAVFAGMEGTRPILVEVQALVAATSLG
TPRRAVVGWDSSRLAMILAVLEAHCGVKLGQHDVYLNVAGGYRISEPAADMAIASALVSSLAGLALPADCVYFGEISLSG
AVRPVSHTGQRLKEAEKLGFSAALLPSASAELPKAGKGRWTEIESLPDLVARIAGSGNRFKQVEDD

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=484732 J8N08_RS03465 WP_003512347.1 704638..706038(-) (radA/sms) [Agrobacterium tumefaciens strain BIM B-441]
ATGGCCAAAGCCAAGACCCAATTCGTGTGCCAGAACTGCGGCACGGTTCATACCCGCTGGGCGGGAAAATGCGAAGGCTG
CGGCGAGTGGAATACCATCGTCGAAGAAGATCCGATGGGCGGCATCGGCTCCGGCCCCGGCAAGACGCCGAAAAAGGGCC
GGGCGGTCGCGCTCACGGCGCTTTCGGGCGAGATCGAGGAGGCGCCGCGCATTCCGACCGGCATCAGCGAACTGGACCGG
GCAACCGGTGGCGGTTTCGTGCGCGGTTCGGCGGTGTTGATCGGCGGCGATCCCGGCATCGGCAAATCGACCCTGCTGAT
GCAGGCGGCAGCTGCCCTTTCGCGGCGCGGACATCGCGTCATCTATGTTTCTGGCGAAGAAGCGGTAGCGCAGGTGCGTC
TGCGCGCCCAGCGGCTGGGTGCGGCGGAAACCGACGTGCTGCTGGCGGCAGAAACCAATGTCGAGGATATTCTGGCGACG
ATTTCCGAGGGCAAACGCCCCGATCTCGTCATCATCGATTCCATCCAGACGCTATGGAGTGACACGGCCGATTCCGCTCC
CGGCACGGTAACGCAGGTGCGCACCGGCGTGCAGGCAATGATCCGTTACGCAAAGCAGACGGGCGCGACCATGGTGCTGG
TGGGCCATGTGACCAAGGAAGGCCAGATCGCCGGTCCGCGCGTGGTGGAGCATATGGTCGACGCCGTGCTTTATTTCGAA
GGCGACCGCGGCCACCATTACCGCATTCTGCGCACCGTCAAGAACCGCTTCGGGCCGACGGACGAGATCGGCGTGTTTGA
AATGTCCGATCGCGGATTGCGCGAGGTCTCAAACCCGTCCGAACTGTTCCTTGGCGAGCGTAATGAGAAATCTCCCGGTG
CCGCGGTCTTTGCCGGCATGGAGGGCACCCGTCCCATTCTGGTCGAGGTGCAGGCGCTGGTCGCGGCCACCTCGCTCGGC
ACGCCGCGCCGCGCCGTGGTCGGCTGGGACAGTTCGCGGCTCGCCATGATCCTTGCCGTGCTGGAAGCCCATTGCGGCGT
GAAGCTCGGTCAGCACGACGTTTATCTGAACGTCGCCGGCGGATACCGCATTTCCGAACCGGCTGCGGATATGGCGATAG
CTTCTGCGCTGGTTTCCTCGCTTGCCGGTCTTGCCCTGCCCGCCGATTGCGTCTATTTCGGCGAGATCAGCCTGTCGGGC
GCGGTACGGCCCGTCTCGCATACGGGCCAGCGTTTGAAAGAAGCTGAAAAACTCGGTTTCTCCGCCGCACTTCTGCCGTC
TGCTTCGGCTGAATTGCCGAAAGCGGGCAAGGGGCGCTGGACGGAAATAGAAAGCCTGCCGGACCTCGTGGCGCGCATCG
CCGGTTCCGGCAACCGGTTCAAACAGGTTGAGGACGACTAA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(343-428)

AAA domain (AAA_25)

(83-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B9V3Y2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

49.774

94.85

0.472
  radA Streptococcus pneumoniae Rx1

46.606

94.85

0.442
  radA Streptococcus pneumoniae D39

46.606

94.85

0.442
  radA Streptococcus pneumoniae R6

46.606

94.85

0.442
  radA Streptococcus pneumoniae TIGR4

46.606

94.85

0.442
  radA Streptococcus mitis SK321

46.774

93.133

0.436
  radA Streptococcus mitis NCTC 12261

46.544

93.133

0.433