Detailed information
Bioinformatically predicted
Overview
| Name | pilS | Type | Regulator |
| Locus tag | IFJ78_RS21895 | Genome accession | NZ_CP061779 |
| Coordinates | 4662048..4663640 (+) | Length | 530 a.a. |
| NCBI ID | WP_003110428.1 | Uniprot ID | - |
| Organism | Pseudomonas aeruginosa strain ZBX-P12 | ||
| Function | regulate pilin expression (predicted from homology) Competence regulation |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 4657654..4724615 | 4662048..4663640 | within | 0 |
Gene organization within MGE regions
Location: 4657654..4724615
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IFJ78_RS21875 (IFJ78_21870) | pgeF | 4658798..4659526 (-) | 729 | WP_003161770.1 | peptidoglycan editing factor PgeF | - |
| IFJ78_RS21880 (IFJ78_21875) | rluD | 4659523..4660485 (-) | 963 | WP_003094686.1 | 23S rRNA pseudouridine(1911/1915/1917) synthase RluD | - |
| IFJ78_RS21885 (IFJ78_21880) | - | 4660631..4661656 (+) | 1026 | WP_003102590.1 | outer membrane protein assembly factor BamD | - |
| IFJ78_RS21890 (IFJ78_21885) | - | 4661813..4662058 (+) | 246 | WP_003102591.1 | PP0621 family protein | - |
| IFJ78_RS21895 (IFJ78_21890) | pilS | 4662048..4663640 (+) | 1593 | WP_003110428.1 | two-component system sensor histidine kinase PilS | Regulator |
| IFJ78_RS21900 (IFJ78_21895) | pilR | 4663655..4664992 (+) | 1338 | WP_003094694.1 | two-component system response regulator PilR | Regulator |
| IFJ78_RS21905 (IFJ78_21900) | thiO | 4665038..4666132 (-) | 1095 | WP_023084775.1 | glycine oxidase ThiO | - |
| IFJ78_RS21910 (IFJ78_21905) | - | 4666267..4666776 (+) | 510 | WP_003161431.1 | Tfp pilus assembly protein FimT/FimU | - |
| IFJ78_RS21915 (IFJ78_21910) | - | 4666882..4667388 (+) | 507 | WP_003102598.1 | Tfp pilus assembly protein FimT/FimU | - |
| IFJ78_RS21920 (IFJ78_21915) | pilV | 4667379..4667936 (+) | 558 | WP_003119423.1 | type 4a pilus minor pilin PilV | Machinery gene |
| IFJ78_RS21925 (IFJ78_21920) | pilW | 4667933..4668757 (+) | 825 | WP_003119422.1 | type 4a pilus minor pilin PilW | - |
| IFJ78_RS21930 (IFJ78_21925) | pilX | 4668754..4669341 (+) | 588 | WP_003112826.1 | type 4a pilus minor pilin PilX | - |
| IFJ78_RS21935 (IFJ78_21930) | pilY1 | 4669353..4672844 (+) | 3492 | WP_003102607.1 | type 4a pilus biogenesis protein PilY1 | - |
| IFJ78_RS21940 (IFJ78_21935) | pilY2 | 4672846..4673193 (+) | 348 | WP_003161774.1 | type 4a fimbrial biogenesis protein PilY2 | - |
| IFJ78_RS21945 (IFJ78_21940) | pilE | 4673190..4673615 (+) | 426 | WP_003094721.1 | type 4a pilus minor pilin PilE | - |
| IFJ78_RS21950 (IFJ78_21945) | ispH | 4673662..4674606 (-) | 945 | WP_003134939.1 | 4-hydroxy-3-methylbut-2-enyl diphosphate reductase | - |
| IFJ78_RS21955 (IFJ78_21950) | fkpB | 4674692..4675132 (-) | 441 | WP_003161775.1 | FKBP-type peptidyl-prolyl cis-trans isomerase | - |
| IFJ78_RS21960 (IFJ78_21955) | lspA | 4675125..4675634 (-) | 510 | WP_003102615.1 | signal peptidase II | - |
| IFJ78_RS21965 (IFJ78_21960) | ileS | 4675627..4678458 (-) | 2832 | WP_003102617.1 | isoleucine--tRNA ligase | - |
| IFJ78_RS21970 (IFJ78_21965) | ribF | 4678482..4679420 (-) | 939 | WP_003110419.1 | bifunctional riboflavin kinase/FAD synthetase | - |
| IFJ78_RS21975 (IFJ78_21970) | murJ | 4679517..4681055 (-) | 1539 | WP_003102622.1 | murein biosynthesis integral membrane protein MurJ | - |
| IFJ78_RS21980 (IFJ78_21975) | rpsT | 4681339..4681614 (+) | 276 | WP_003094744.1 | 30S ribosomal protein S20 | - |
| IFJ78_RS21985 (IFJ78_21980) | - | 4681681..4682145 (-) | 465 | WP_003094746.1 | CreA family protein | - |
| IFJ78_RS21990 (IFJ78_21985) | proB | 4682157..4683275 (-) | 1119 | WP_003094756.1 | glutamate 5-kinase | - |
| IFJ78_RS21995 (IFJ78_21990) | cgtA | 4683346..4684566 (-) | 1221 | WP_003094758.1 | Obg family GTPase CgtA | - |
| IFJ78_RS22000 (IFJ78_21995) | - | 4684957..4685202 (-) | 246 | WP_000108283.1 | DUF2274 domain-containing protein | - |
| IFJ78_RS22005 (IFJ78_22000) | - | 4685199..4686479 (-) | 1281 | WP_003092274.1 | TrbI/VirB10 family protein | - |
| IFJ78_RS22010 (IFJ78_22005) | trbG | 4686482..4687474 (-) | 993 | WP_000776560.1 | P-type conjugative transfer protein TrbG | - |
| IFJ78_RS22015 (IFJ78_22010) | trbF | 4687471..4688175 (-) | 705 | WP_001211312.1 | conjugal transfer protein TrbF | - |
| IFJ78_RS22020 (IFJ78_22015) | trbL | 4688194..4689561 (-) | 1368 | WP_001000746.1 | P-type conjugative transfer protein TrbL | - |
| IFJ78_RS22025 (IFJ78_22020) | - | 4689558..4689884 (-) | 327 | WP_014603538.1 | hypothetical protein | - |
| IFJ78_RS22030 (IFJ78_22025) | trbJ | 4689897..4690640 (-) | 744 | WP_003092277.1 | P-type conjugative transfer protein TrbJ | - |
| IFJ78_RS22035 (IFJ78_22030) | trbE | 4690637..4693087 (-) | 2451 | WP_000933135.1 | conjugal transfer protein TrbE | - |
| IFJ78_RS22040 (IFJ78_22035) | - | 4693100..4693369 (-) | 270 | WP_003092280.1 | VirB3 family type IV secretion system protein | - |
| IFJ78_RS22045 (IFJ78_22040) | - | 4693366..4693752 (-) | 387 | WP_000150010.1 | TrbC/VirB2 family protein | - |
| IFJ78_RS22050 (IFJ78_22045) | trbB | 4693749..4694819 (-) | 1071 | WP_023093367.1 | P-type conjugative transfer ATPase TrbB | - |
| IFJ78_RS22055 (IFJ78_22050) | - | 4694816..4695292 (-) | 477 | WP_000085131.1 | ribbon-helix-helix protein, CopG family | - |
| IFJ78_RS22060 (IFJ78_22055) | - | 4695289..4697286 (-) | 1998 | WP_001163195.1 | IncP-type conjugal transfer protein TraG | - |
| IFJ78_RS22065 (IFJ78_22060) | - | 4697592..4698497 (+) | 906 | WP_023093368.1 | LysR family transcriptional regulator | - |
| IFJ78_RS22070 (IFJ78_22065) | - | 4698779..4699309 (+) | 531 | WP_000043569.1 | hypothetical protein | - |
| IFJ78_RS22075 (IFJ78_22070) | - | 4699411..4699590 (+) | 180 | Protein_4382 | integrase core domain-containing protein | - |
| IFJ78_RS22080 (IFJ78_22075) | - | 4699819..4700136 (-) | 318 | WP_000539741.1 | multidrug efflux SMR transporter | - |
| IFJ78_RS22085 (IFJ78_22080) | - | 4700195..4700458 (-) | 264 | WP_000717310.1 | EexN family lipoprotein | - |
| IFJ78_RS22090 (IFJ78_22085) | - | 4700491..4701396 (-) | 906 | WP_000405670.1 | LysR family transcriptional regulator | - |
| IFJ78_RS22095 (IFJ78_22090) | - | 4701490..4702920 (-) | 1431 | WP_249225083.1 | TolC family protein | - |
| IFJ78_RS22100 (IFJ78_22095) | - | 4702949..4703977 (+) | 1029 | WP_050161940.1 | IS30-like element ISPsp8 family transposase | - |
| IFJ78_RS22105 (IFJ78_22100) | - | 4704082..4704750 (+) | 669 | WP_143485411.1 | TetR family transcriptional regulator | - |
| IFJ78_RS22110 (IFJ78_22105) | - | 4704771..4705913 (+) | 1143 | WP_223294861.1 | efflux RND transporter periplasmic adaptor subunit | - |
| IFJ78_RS22115 (IFJ78_22110) | - | 4705938..4709042 (+) | 3105 | WP_003092293.1 | efflux RND transporter permease subunit | - |
| IFJ78_RS22120 (IFJ78_22115) | - | 4709123..4709470 (+) | 348 | WP_038421338.1 | SMR family transporter | - |
| IFJ78_RS22125 (IFJ78_22120) | - | 4709486..4710106 (+) | 621 | WP_000837791.1 | DUF3313 family protein | - |
| IFJ78_RS22130 (IFJ78_22125) | - | 4710103..4711260 (+) | 1158 | Protein_4393 | ABC transporter permease | - |
| IFJ78_RS22135 (IFJ78_22130) | - | 4711257..4712078 (+) | 822 | WP_023093371.1 | ATP-binding cassette domain-containing protein | - |
| IFJ78_RS22140 (IFJ78_22135) | - | 4712170..4713099 (+) | 930 | WP_000433686.1 | MlaD family protein | - |
| IFJ78_RS22145 (IFJ78_22140) | - | 4713192..4713791 (+) | 600 | WP_003124411.1 | ABC-type transport auxiliary lipoprotein family protein | - |
| IFJ78_RS22150 (IFJ78_22145) | - | 4713858..4715861 (-) | 2004 | WP_000132148.1 | relaxase/mobilization nuclease and DUF3363 domain-containing protein | - |
| IFJ78_RS22155 (IFJ78_22150) | - | 4716310..4716897 (-) | 588 | WP_003092304.1 | S26 family signal peptidase | - |
| IFJ78_RS22160 (IFJ78_22155) | - | 4716894..4717451 (-) | 558 | WP_023093372.1 | DUF2840 domain-containing protein | - |
| IFJ78_RS22165 (IFJ78_22160) | - | 4717448..4717711 (-) | 264 | WP_000147048.1 | hypothetical protein | - |
| IFJ78_RS22170 (IFJ78_22165) | parA | 4717708..4718346 (-) | 639 | WP_003092312.1 | ParA family partition ATPase | - |
| IFJ78_RS22175 (IFJ78_22170) | - | 4718588..4719433 (-) | 846 | WP_022652188.1 | replication initiator protein A | - |
| IFJ78_RS22180 (IFJ78_22175) | - | 4719460..4719744 (-) | 285 | WP_001247107.1 | helix-turn-helix domain-containing protein | - |
| IFJ78_RS22185 (IFJ78_22180) | - | 4719856..4720626 (-) | 771 | WP_003092318.1 | DUF2285 domain-containing protein | - |
| IFJ78_RS22190 (IFJ78_22185) | - | 4720969..4721316 (-) | 348 | WP_016487838.1 | DUF2958 domain-containing protein | - |
| IFJ78_RS22195 (IFJ78_22190) | - | 4721604..4721897 (+) | 294 | WP_006379425.1 | helix-turn-helix transcriptional regulator | - |
| IFJ78_RS22200 (IFJ78_22195) | - | 4722210..4722524 (-) | 315 | WP_001273857.1 | DUF736 domain-containing protein | - |
| IFJ78_RS22205 (IFJ78_22200) | - | 4723300..4723509 (-) | 210 | WP_022580212.1 | hypothetical protein | - |
Sequence
Protein
Download Length: 530 a.a. Molecular weight: 58986.14 Da Isoelectric Point: 6.5650
>NTDB_id=484677 IFJ78_RS21895 WP_003110428.1 4662048..4663640(+) (pilS) [Pseudomonas aeruginosa strain ZBX-P12]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS
Nucleotide
Download Length: 1593 bp
>NTDB_id=484677 IFJ78_RS21895 WP_003110428.1 4662048..4663640(+) (pilS) [Pseudomonas aeruginosa strain ZBX-P12]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTACTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTTGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATATCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTACTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTTGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATATCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilS | Pseudomonas aeruginosa PAK |
99.811 |
100 |
0.998 |