Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J8I92_RS02090 Genome accession   NZ_CP072688
Coordinates   440960..441637 (-) Length   225 a.a.
NCBI ID   WP_000178172.1    Uniprot ID   -
Organism   Escherichia coli strain P4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435960..446637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8I92_RS02075 (J8I92_02035) chiA 437321..440020 (+) 2700 WP_000773163.1 bifunctional chitinase/lysozyme -
  J8I92_RS02080 (J8I92_02040) bfd 440189..440383 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J8I92_RS02085 (J8I92_02045) bfr 440455..440931 (+) 477 WP_000675504.1 bacterioferritin -
  J8I92_RS02090 (J8I92_02050) pilD 440960..441637 (-) 678 WP_000178172.1 prepilin peptidase GspO Machinery gene
  J8I92_RS02095 (J8I92_02055) gspM 441637..442098 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  J8I92_RS02100 (J8I92_02060) gspL 442095..443258 (-) 1164 WP_001304915.1 type II secretion system protein GspL -
  J8I92_RS02105 (J8I92_02065) gspK 443273..444256 (-) 984 WP_001058529.1 type II secretion system minor pseudopilin GspK -
  J8I92_RS02110 (J8I92_02070) gspJ 444249..444836 (-) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  J8I92_RS02115 (J8I92_02075) gspI 444829..445206 (-) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  J8I92_RS02120 (J8I92_02080) gspH 445203..445712 (-) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  J8I92_RS02125 (J8I92_02085) gspG 445720..446157 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.70 Da        Isoelectric Point: 7.6364

>NTDB_id=484646 J8I92_RS02090 WP_000178172.1 440960..441637(-) (pilD) [Escherichia coli strain P4]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTAPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=484646 J8I92_RS02090 WP_000178172.1 440960..441637(-) (pilD) [Escherichia coli strain P4]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGCGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382