Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ID616_RS25470 Genome accession   NZ_CP061723
Coordinates   5583779..5585149 (-) Length   456 a.a.
NCBI ID   WP_016489099.1    Uniprot ID   A0A1L7NJL7
Organism   Pseudomonas putida strain ZXPA-20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5578779..5590149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ID616_RS25450 (ID616_25450) - 5579001..5579198 (-) 198 WP_003251633.1 YbdD/YjiX family protein -
  ID616_RS25455 (ID616_25455) - 5579216..5581282 (-) 2067 WP_016489096.1 carbon starvation CstA family protein -
  ID616_RS25460 (ID616_25460) - 5581460..5581825 (+) 366 WP_023047384.1 PilZ domain-containing protein -
  ID616_RS25465 (ID616_25465) - 5582089..5583606 (+) 1518 WP_016489098.1 nucleobase:cation symporter-2 family protein -
  ID616_RS25470 (ID616_25470) radA 5583779..5585149 (-) 1371 WP_016489099.1 DNA repair protein RadA Machinery gene
  ID616_RS25475 (ID616_25475) mscL 5585326..5585745 (+) 420 WP_016489100.1 large-conductance mechanosensitive channel protein MscL -
  ID616_RS25480 (ID616_25480) - 5585779..5586555 (-) 777 WP_024718028.1 ferredoxin--NADP reductase -
  ID616_RS25485 (ID616_25485) - 5586768..5587475 (+) 708 WP_191086971.1 autoinducer binding domain-containing protein -
  ID616_RS25490 (ID616_25490) - 5587572..5588696 (+) 1125 WP_023047385.1 methyltransferase -
  ID616_RS25495 (ID616_25495) - 5588871..5589026 (-) 156 WP_016489104.1 DUF2474 domain-containing protein -
  ID616_RS25500 (ID616_25500) cydB 5589036..5590043 (-) 1008 WP_016489105.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 48729.17 Da        Isoelectric Point: 7.1315

>NTDB_id=484404 ID616_RS25470 WP_016489099.1 5583779..5585149(-) (radA) [Pseudomonas putida strain ZXPA-20]
MAKAKRLYGCTECGATFPKWAGQCGECGAWNTLVETMIESGGAAAPSSGRAGWTGQQAQIKTLAEVSVEEIPRFTTSSTE
LDRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIASGMPALYVTGEESQQQVAMRSRRLGLPQDKLKVMTETCIETII
ATARVEKPRVMVIDSIQTIFTEQLQSAPGGVAQVRESTALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYF
EGESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRTQEEVPGSVVMATWEGTRPMLVEVQALVDDSHL
ANPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALLAAVMSSLRNRPLAHGLLVFGEIGLS
GEVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPAGLQVIAVTRLEQALDALFE

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=484404 ID616_RS25470 WP_016489099.1 5583779..5585149(-) (radA) [Pseudomonas putida strain ZXPA-20]
ATGGCCAAGGCCAAGCGCTTGTATGGCTGCACCGAGTGCGGCGCGACCTTCCCCAAATGGGCCGGCCAGTGTGGCGAATG
CGGAGCCTGGAACACGCTGGTCGAAACCATGATCGAAAGCGGTGGTGCGGCTGCGCCCAGCAGCGGCCGCGCCGGCTGGA
CCGGCCAGCAAGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTTGAGGAAATCCCGCGCTTCACCACCAGCAGCACCGAA
CTGGACCGCGTGTTGGGCGGTGGCCTGGTGGATGGCTCGGTGGTGCTGATTGGTGGCGACCCAGGCATCGGCAAATCGAC
CATCCTGCTGCAGACCTTGTGCAATATTGCCTCGGGCATGCCGGCGCTGTACGTCACGGGTGAAGAGTCGCAGCAGCAAG
TGGCCATGCGCTCGCGGCGCCTGGGCTTGCCCCAGGACAAGCTCAAGGTCATGACCGAAACCTGTATTGAAACCATTATC
GCCACCGCGCGGGTCGAGAAGCCTCGGGTAATGGTGATCGACTCGATCCAGACTATTTTCACCGAGCAGTTGCAGTCGGC
TCCTGGCGGGGTAGCCCAGGTGCGCGAGAGCACGGCGCTACTGGTGCGTTACGCCAAGCAGAGCGGCACGGCGATCTTCC
TGGTCGGCCACGTGACCAAGGAAGGCTCGCTGGCCGGCCCACGGGTACTGGAGCACATGGTCGATACCGTGCTGTATTTC
GAAGGTGAGTCCGACGGCCGCCTGCGCTTGTTGCGAGCGGTGAAAAACCGCTTTGGTGCGGTCAACGAACTGGGCGTGTT
CGGCATGACCGACCGGGGCCTGAAAGAGGTGTCCAACCCGTCGGCGATCTTCCTTAACCGTACCCAGGAAGAAGTACCGG
GCAGTGTGGTGATGGCGACTTGGGAAGGCACCCGGCCAATGCTGGTCGAGGTACAGGCGCTGGTCGACGACAGCCACTTG
GCCAACCCGCGCCGGGTAACCCTGGGCCTGGACCAGAACCGCCTGGCCATGTTGCTGGCGGTGCTGCACCGCCACGGCGG
TATTCCCACCCACGATCAGGACGTGTTCTTAAATGTGGTAGGCGGGGTCAAGGTGCTGGAAACGGCGTCGGACCTGGCGC
TTCTGGCGGCAGTGATGTCCAGCTTGCGTAACCGGCCACTGGCCCATGGGCTGCTGGTGTTTGGCGAGATCGGCCTGTCG
GGCGAGGTGCGGCCGGTGCCTAGCGGGCAGGAGCGGTTGAAGGAAGCGGCCAAGCATGGCTTCAAGCGGGCGATCGTGCC
CAAGGGCAATGCGCCGAAAGAGGCGCCGGCGGGGCTGCAGGTAATTGCCGTCACCCGTCTGGAACAGGCCCTGGATGCAT
TGTTCGAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(73-222)

Subunit ChlI of Mg-chelatase (ChlI)

(355-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L7NJL7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.904

100

0.489
  radA Streptococcus pneumoniae Rx1

46.087

100

0.465
  radA Streptococcus pneumoniae D39

46.087

100

0.465
  radA Streptococcus pneumoniae TIGR4

46.087

100

0.465
  radA Streptococcus pneumoniae R6

46.087

100

0.465
  radA Streptococcus mitis NCTC 12261

45.87

100

0.463
  radA Streptococcus mitis SK321

46.053

100

0.461