Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   J7886_RS02635 Genome accession   NZ_CP072539
Coordinates   602014..602691 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli O128ac:H12 strain AW1.7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 597014..607691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J7886_RS02620 (J7886_02620) chiA 598381..601074 (+) 2694 WP_000773174.1 bifunctional chitinase/lysozyme -
  J7886_RS02625 (J7886_02625) bfd 601243..601437 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  J7886_RS02630 (J7886_02630) bfr 601509..601985 (+) 477 WP_000675504.1 bacterioferritin -
  J7886_RS02635 (J7886_02635) pilD 602014..602691 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  J7886_RS02640 (J7886_02640) gspM 602691..603152 (-) 462 WP_001549764.1 type II secretion system protein GspM -
  J7886_RS02645 (J7886_02645) gspL 603149..604312 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  J7886_RS02650 (J7886_02650) gspK 604327..605310 (-) 984 Protein_533 type II secretion system minor pseudopilin GspK -
  J7886_RS02655 (J7886_02655) gspJ 605303..605890 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  J7886_RS02660 (J7886_02660) gspI 605883..606260 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  J7886_RS02665 (J7886_02665) gspH 606257..606766 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  J7886_RS02670 (J7886_02670) gspG 606774..607211 (-) 438 WP_054626323.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=483992 J7886_RS02635 WP_000178161.1 602014..602691(-) (pilD) [Escherichia coli O128ac:H12 strain AW1.7]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=483992 J7886_RS02635 WP_000178161.1 602014..602691(-) (pilD) [Escherichia coli O128ac:H12 strain AW1.7]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378