NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	IC771_RS20740	Genome accession	NZ_CP061666
Coordinates	340027..340887 (-)	Length	286 a.a.
NCBI ID	WP_077169131.1	Uniprot ID	A0A7H2QYC9
Organism	Acinetobacter seifertii strain AS25
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 335027..345887

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC771_RS20770	-	336793..337228 (+)	436	Protein_307	tetratricopeptide repeat protein	-
IC771_RS01605 (IC771_01600)	-	337305..337631 (+)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
IC771_RS01610 (IC771_01605)	rlmB	337734..338483 (+)	750	WP_039251214.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
IC771_RS01615 (IC771_01610)	-	338518..339432 (+)	915	WP_077169133.1	DMT family transporter	-
IC771_RS01620 (IC771_01615)	coaE	339429..340025 (-)	597	WP_077169132.1	dephospho-CoA kinase	-
IC771_RS20740 (IC771_01620)	pilD	340027..340887 (-)	861	WP_077169131.1	prepilin peptidase	Machinery gene
IC771_RS01630 (IC771_01625)	pilC	340887..342113 (-)	1227	WP_077169130.1	type II secretion system F family protein	Machinery gene
IC771_RS01635 (IC771_01630)	pilB	342141..343853 (-)	1713	WP_077169129.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IC771_RS01640 (IC771_01635)	tpiA	344143..344937 (+)	795	WP_151731148.1	triose-phosphate isomerase	-
IC771_RS01645 (IC771_01640)	secG	344950..345279 (+)	330	WP_019456770.1	preprotein translocase subunit SecG	-

Sequence

Protein

Download Length: 286 a.a. Molecular weight: 32092.52 Da Isoelectric Point: 7.1785

                                >NTDB_id=483992 IC771_RS20740 WP_077169131.1 340027..340887(-) (pilD) [Acinetobacter seifertii strain AS25]

MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPNCHQP

IRWYQNIPVISWLMLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMVFGPTIQMLLGLVLTWVLIALTFIDFDTQLLPDR

FTLPLAALGLGINTFNIYTAPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL

LGAITGIILLKLRNENQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

Download Length: 861 bp

                                >NTDB_id=483992 IC771_RS20740 WP_077169131.1 340027..340887(-) (pilD) [Acinetobacter seifertii strain AS25]

ATGCAAGATATTATTGCTTATTTTATTCAAAATTTAACCGCACTTTATATTGCAGTTGCACTCGTGAGCCTATGTATTGG

TAGCTTTCTAAATGTAGTGATTTATCGCACTCCTAAAATGATGGAACAAGATTGGCAGCAAGAATGTCAAATGTTACTCA

ATCCTGAACAACCAATCATTGATCATGAAAAGCTAACATTAAGTAAACCTGCTTCATCATGTCCAAATTGCCATCAACCG

ATTCGTTGGTATCAAAATATCCCAGTCATAAGCTGGTTGATGTTAAGAGGAAAATGTGGTCATTGCCAACATCCTATCAG

CATTCGTTATCCAGCAATTGAACTACTCACCATGTTATGTTCATTGGTGGTGGTTATGGTGTTTGGCCCGACCATACAAA

TGCTTTTGGGTCTCGTCCTGACTTGGGTTTTAATTGCCCTCACCTTTATAGACTTTGATACACAACTTTTACCTGATCGC

TTTACCCTACCTTTGGCAGCACTTGGCTTGGGCATTAACACCTTTAATATTTATACCGCGCCTAACTCAGCTATTTGGGG

TTATTTAATCGGCTTTTTATGTCTCTGGATTGTGTATTACTTATTTAAAGTGATTACAGGTAAAGAAGGTATGGGCTATG

GCGACTTTAAGTTACTTGCAGCATTGGGCGCATGGATGGGGCCATTGATGCTGCCATTAATTGTGTTATTGTCATCTTTA

TTGGGTGCAATTACTGGCATCATTTTATTAAAGTTAAGAAATGAAAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT

TGCGGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAGATTTATTTAGGAGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-128)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A7H2QYC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Acinetobacter nosocomialis M2	97.203	100	0.972
pilD	Acinetobacter baumannii D1279779	96.853	100	0.969
pilD	Vibrio cholerae strain A1552	53.48	95.455	0.51
pilD	Vibrio campbellii strain DS40M4	52.399	94.755	0.497
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441

Detailed information NTG0483992